Probe:	Hox2.3 probe4
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		1A/B
TS11: primitive streak	Present	Regionally restricted	1A/B	Expression was detected within and along the posterior part of the primitive streak in ectoderm and mesoderm.

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		1C/D
TS11: primitive streak	Present	Regionally restricted	1C/D	Expression was detected in ectoderm and mesoderm of the primitive streak. The expression domain spread to reach the anterior part of the primitive streak. The rostral extremity of expression was further anterior in the ectoderm than in the mesoderm.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	1E/F	The rostral limit of expression is in neurectoderm located more anteriorly than the node.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1G/H	The rostral expression boundary in the neurectoderm was at the level of the 5th somite.
TS13: trunk unsegmented mesenchyme	Present		1G/H	The rostral boundary of expression in the presomitic mesoderm was at a more posterior position than the 5th somite.

Structure	Level	Pattern	Image	Note
TS15: spinal ganglion	Present		1I/J
TS15: future hindbrain	Present	Regionally restricted	1I/J	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord.
TS15: future spinal cord	Present	Regionally restricted	1I/J	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord.
TS15: somite 11	Present		1I/J	Expression in mesoderm extended from somite 11 down to the tail bud.
TS15: tail mesenchyme	Present		1I/J	Expression in mesoderm extended from somite 11 down to the tail bud.
TS15: trunk paraxial mesenchyme	Present		1I/J	Expression in mesoderm extended from somite 11 down to the tail bud.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	1K/L	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord at the level of the 5th somite.
TS20: spinal cord	Present	Regionally restricted	1K/L	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord at the level of the 5th somite.
TS20: vertebral pre-cartilage condensation	Present	Regionally restricted	1K/L	The rostral boundary of expression was at the level of the eighth prevertebra.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1M/N	Preferential accumulation of transcripts was in the spinal cord dorsal horns.
TS20: spinal ganglion	Weak		1M/N
TS20: vertebral pre-cartilage condensation	Weak		1M/N
TS20: gland	Weak	Regionally restricted	1M/N	Lower expression was detected in the adrenal gland.