Probe:	Cad6 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	2A	Expression is in an anterior crescent area.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		2B

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	2C	Expression is at the boundary.
TS12: future prosencephalon	Present	Regionally restricted	2C	Expression is at the boundary.
TS12: future hindbrain	Present		2C
TS12: future midbrain neural crest	Present		2C

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	2D	Expression is at the boundary.
TS12: future prosencephalon	Present	Regionally restricted	2D	Expression is at the boundary.
TS12: future hindbrain	Present		2D
TS12: future midbrain neural crest	Present		2D
TS12: future spinal cord	Present	Regionally restricted	2D	Expression is at the caudal end.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	2E	Expression is at the ridge of the neural fold.
TS12: future hindbrain	Present	Regionally restricted	2E	Expression is at the ridge of the neural fold of the anterior hindbrain.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		2F

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		2G
TS13: future hindbrain	Present	Regionally restricted	2G	Expression is in a band-like pattern.

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		2H

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		2I
TS13: future hindbrain	Present	Regionally restricted	2I	Expression is in a band-like pattern.

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		2J

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	3A	The anterior limit of expression is the rhombomere 4/5 boundary and is virtually negative in rhombomere 3 and 5.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	3B	Rhombomere 6.
TS13: future hindbrain	Trace	Regionally restricted	3B	Virtually negative in rhombomere 3 and 5.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present		4A
TS14: rhombomere 6	Present		4A

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present		4B
TS15: cranial preganglion	Present		4B
TS15: optic stalk	Present		4B
TS15: rhombomere 6	Present		4B
TS15: trigeminal preganglion	Present		4B
TS15: trunk mesenchyme derived from neural crest	Present		4B
TS15: future hindbrain	Present	Regionally restricted	4B	Expression is at the anterior border of the dorsal hindbrain.
TS15: future midbrain	Present	Regionally restricted	4B	Expression is in a dorsal portion.
TS15: diencephalon	Present	Regionally restricted	4B
TS15: future forebrain	Present	Regionally restricted	4B
TS15: eye	Present	Regionally restricted	4B	Future neural retina.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	4C	Expression is at the anterior border of the dorsal hindbrain.
TS15: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present		4C
TS15: trigeminal preganglion	Present		4C

Structure	Level	Pattern	Image	Note
TS20: hypothalamus ventricular layer	Present		4D
TS20: diencephalon ventricular layer	Weak	Regionally restricted	4D	As a line at the zona limitans intrathalamica along the boundary between the dorsal and ventral thalamus and along the alar/basal boundary in the diencephalon and midbrain.
TS20: midbrain ventricular layer	Weak	Regionally restricted	4D	As a line at the zona limitans intrathalamica along the boundary between the dorsal and ventral thalamus and along the alar/basal boundary in the diencephalon and midbrain.
TS20: thalamus ventricular layer	Weak	Regionally restricted	4D	As a line at the zona limitans intrathalamica along the boundary between the dorsal and ventral thalamus and along the alar/basal boundary in the diencephalon and midbrain.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	4E	Expression is in a dorsal portion.

Structure	Level	Pattern	Image	Note
TS20: nerve	Present		5A
TS20: spinal cord floor plate	Present		5A
TS20: spinal cord roof plate	Present		5A
TS20: sympathetic ganglion	Present	Regionally restricted	5A	Expression is in the peripheral zone of the sympathetic ganglia.
TS20: basal columns	Present	Regionally restricted	5A	Expression is in a portion.
TS20: tail mesenchyme derived from neural crest	Present	Regionally restricted	5A	Expression is in neural crest-derived cells around the trachea, artery, and esophagus.

Structure	Level	Pattern	Image	Note
TS20: basal columns	Present	Regionally restricted	5B
TS20: spinal cord floor plate	Present	Regionally restricted	5B
TS20: spinal cord roof plate	Present	Regionally restricted	5B
TS20: nerve	Present		5B , 5C
TS20: dorsal root ganglion	Absent		5B

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present		5E
TS20: spinal cord floor plate	Present		5E
TS20: spinal cord roof plate	Present		5E
TS20: basal columns	Present	Regionally restricted	5E

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	5F	Expression is at the edges of the open neural plate.
TS20: hindlimb	Absent		5F

Structure	Level	Pattern	Image	Note
TS20: nerve	Present		5G
TS20: hindlimb interdigital region	Present	Regionally restricted	5G	Expression is beneath the epidermis in a nerve-like network.

Structure	Level	Pattern	Image	Note
TS23: peripheral nerve	Absent		5H

Structure	Level	Pattern	Image	Note
TS20: eye	Present	Regionally restricted		Future iris.
TS20: optic stalk	Present

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	6B +/-

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	6B -/-	The anterior limit of expression has retreated compared to wild-type.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Present		6C
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	6C	Rhombomere 6.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Weak		6D
TS13: future hindbrain posterior to rhombomere 5	Weak	Regionally restricted	6D	Rhombomere 6.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 2	Present		6E
TS13: rhombomere 4	Present		6E
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	6E	Rhombomere 6.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Weak		6F
TS13: future hindbrain posterior to rhombomere 5	Weak	Regionally restricted	6F	Rhombomere 6.
TS13: rhombomere 2	Present		6F	Expression extends more caudally in this mutant.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Present	Regionally restricted	6G	At the neural fold ridge.
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	6G	Rhombomere 6 at the neural fold ridge.
TS13: rhombomere 2	Present		6G

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Present		6H	Expression was basically normal in this mutant.
TS13: rhombomere 4	Present		6H	Expression was basically normal in this mutant.
TS13: rhombomere 2	Present		6H	Expression extends more caudally in this mutant.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		6I
TS15: rhombomere 4	Present		6I
TS15: rhombomere 6	Present		6I

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		6J	Expression is similar to wild-type.
TS15: rhombomere 4	Present		6J	Expression is similar to wild-type.
TS15: rhombomere 6	Present		6J	Expression is similar to wild-type.