Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51099
Asakura A, et al., Apoptosis of epaxial myotome in Danforth's short-tail (Sd) mice in somites that form following notochord degeneration. Dev Biol. 1998 Nov 15;203(2):276-89
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5489066
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Gene symbol:
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Myh3
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Gene name:
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myosin, heavy polypeptide 3, skeletal muscle, embryonic
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Probe:
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MHC probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Embryos were fixed in glutaraldehyde/paraformaldehyde (0.2%/0.4%).
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Results
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Specimen
4A:
embryonic day 10.0
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Widespread |
4A
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Expression in entire myotome in rostral to caudal regions.
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| TS16: somite |
Present |
Regionally restricted |
4A
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Expresssion restricted at center of newly formed somites in tailbud region.
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Specimen
4B:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Regionally restricted |
4B
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Expression detected in entire myotome in rostral regions, but significantly reduced in dorsal part of myotome in caudal regions.
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Specimen
4C:
embryonic day 10.0
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: tail somite |
Present |
Regionally restricted |
4C
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Expression restricted at center of newly formed somites in tailbud region.
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Specimen
4D:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: tail somite |
Present |
Regionally restricted |
4D
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Expression restricted at center of newly formed myotome in tailbud region.
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Specimen
4E:
embryonic day 10.0
(more )
Specimen
4E:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Widespread |
4E
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Expression in entire myotome in rostral to caudal regions.
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Specimen
4F:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Regionally restricted |
4F
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Expression significantly reduced in dorsal part of myotome in caudal regions.
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Specimen
6E:
embryonic day 11.5
(more )
Specimen
6E:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
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6E
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| TS19: face |
Present |
Regionally restricted |
6E
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Expression in skeletal muscle of forelimb and hindlimb buds, and in facial muscle.
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| TS19: forelimb bud |
Present |
Regionally restricted |
6E
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Expression in skeletal muscle of forelimb and hindlimb buds, and in facial muscle.
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| TS19: hindlimb bud |
Present |
Regionally restricted |
6E
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Expression in skeletal muscle of forelimb and hindlimb buds, and in facial muscle.
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Specimen
6F:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
6F
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Expression in entire myotome in rostral region, but significantly decreased in dorsal myotome in caudal region.
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| TS19: face |
Present |
Regionally restricted |
6F
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Expression in forelimb bud, hindlimb bud and facial muscles not significantly affected compare to wildtype.
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| TS19: forelimb bud |
Present |
Regionally restricted |
6F
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Expression in forelimb bud, hindlimb bud and facial muscles not significantly affected compare to wildtype.
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| TS19: hindlimb bud |
Present |
Regionally restricted |
6F
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Expression in forelimb bud, hindlimb bud and facial muscles not significantly affected compare to wildtype.
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Specimen
6G:
embryonic day 11.5
(more )
Specimen
6G:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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Specimen
6H:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
6H
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Expression in entire myotome in rostral region, but significantly decreased in dorsal myotome in caudal region.
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