Gene Expression Data
Assay Details
Assay
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Reference:
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J:104355
Sugiyama-Nakagiri Y, et al., Expression of RNA-Binding Protein Musashi in Hair Follicle Development and Hair Cycle Progression. Am J Pathol. 2006 Jan;168(1):80-92
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5478588
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Gene symbol:
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Msi1
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Gene name:
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musashi RNA-binding protein 1
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Antibody:
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anti-Msi1
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2D:
embryonic day 14.5
(more )
Specimen
2D:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: epidermis |
Present |
Widespread |
2D
|
|
TS22: hair follicle |
Present |
Regionally restricted |
2D
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Expressed in hair germ and bulbous hair peg.
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TS22: dermis |
Present |
Regionally restricted |
2D
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Expressed in the dermal cells surrounding the hair placode and in the upper dermis.
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Specimen
2E:
embryonic day 15.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: hair follicle |
Present |
Regionally restricted |
2E
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Expressed in hair germ and bulbous hair peg. Strong expression in the inner cells of the hair germ.
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Specimen
2F:
embryonic day 18.5
(more )
Specimen
2F:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: hair bulb |
Strong |
Widespread |
2F
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Highly expressed throughout the entire bulbous hair peg.
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TS26: epidermis basal layer |
Strong |
Widespread |
2F
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Highly expressed throughout interfollicular epidermal basal layer.
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Specimen
3A:
postnatal day 1
(more )
Specimen
3A:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal day 1
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3B:
postnatal day 4
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 4
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3C:
postnatal day 8
(more )
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 8
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
3D:
postnatal day 11
(more )
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 11
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
3E:
postnatal day 18
(more )
Specimen
3E:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 18
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
3F:
postnatal day 21
(more )
Specimen
3F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 21
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair bulb |
Present |
|
3F
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Expressed in the nucleus of the bulge and the secondary germ cells.
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TS28: epidermis |
Present |
|
3F
|
|
Specimen
9A:
postnatal day 5
(more )
Specimen
9A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair inner root sheath |
Present |
Regionally restricted |
9A
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Expressed in the outermost layer in the middle of the hair follicle.
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Specimen
9B:
postnatal day 19
(more )
Specimen
9B:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 19
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair inner root sheath |
Present |
Regionally restricted |
9B
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Expressed in the outermost layer in the middle of the hair follicle.
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