Antibody:	3F11
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: embryo	Present

Structure	Level	Pattern	Image	Note
TS17: brain	Strong		2B	Most prominent expression in immature neural tissue of brain and spinal cord.
TS17: future spinal cord	Strong		2B	Most prominent expression in immature neural tissue of brain and spinal cord.
TS17: 1st branchial arch	Present		2B
TS17: gut	Present		2B
TS17: somite	Present		2B
TS17: embryo mesenchyme	Present	Regionally restricted	2B	No expression loose connective tissue areas.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		2C
TS20: forebrain	Strong		2C
TS20: gut	Strong		2C
TS20: hindbrain	Strong		2C
TS20: lung	Strong		2C
TS20: metanephros	Strong		2C
TS20: head mesenchyme	Weak		2C	Expression was noticeably lower in the liver and in connective tissues of the head and body.
TS20: liver	Weak		2C	Expression was noticeably lower in the liver and in connective tissues of the head and body.
TS20: trunk mesenchyme	Weak		2C	Expression was noticeably lower in the liver and in connective tissues of the head and body.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present

Structure	Level	Pattern	Image	Note
TS22: embryo	Strong	Widespread	2D	Expression was widespread and intense, but not ubiquitous.
TS22: brain	Present	Non-Uniform	2D
TS22: cortical plate	Strong		2D
TS22: brain ventricular layer	Present		2D
TS22: gut	Present		2D
TS22: hindlimb	Present		2D
TS22: pituitary gland	Present		2D
TS22: vibrissa follicle	Present		2D
TS22: liver	Absent		2D
TS22: thalamus	Absent		2D

Structure	Level	Pattern	Image	Note
TS24: nasal cavity epithelium	Present		2E
TS24: spinal cord	Present		2E
TS24: metanephros	Present	Graded	2E	Gradient of expression, with highest levels in immature cap tissue near surface.
TS24: lung	Present	Non-Uniform	2E
TS24: hair follicle	Present		2E

Structure	Level	Pattern	Image	Note
TS26: embryo	Weak		2F	Decreased overall levels of expression.
TS26: brain	Present	Regionally restricted	2F
TS26: cortical plate	Weak		2F	Overall staining intensity decreased as cells differentiated.
TS26: nasal cavity epithelium	Strong		2F
TS26: tongue epithelium	Present		2F
TS26: incisor	Strong	Regionally restricted	2F	Expression in ameloblasts and odontoblast of incisor bud.
TS26: thymus	Present		2F

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong		3A	Expression in most cells over both nucleus and cytoplasm.

Structure	Level	Pattern	Image	Note
TS26: inner ear	Strong	Regionally restricted	4A	Expression is high in sensory hair cells. Lower levels of expression in supporting tissues.
TS26: cochlear ganglion	Strong		4A

Structure	Level	Pattern	Image	Note
TS24: nasal cavity epithelium	Present	Regionally restricted	4B	Expression in more intense in differentiated receptors than in the proliferating stem cell layer.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	5A	No expression in the vasculature on the inside surface of the retina.
TS24: neural retina	Present	Uniform	5A	Uniform expression in the neuroepithelium except for scattered cells adjacent to the pigment epithelium.

Structure	Level	Pattern	Image	Note
TS26: neural retina	Present	Graded		Gradient of expression, with lower levels in cells close to the retinal pigmented epithelium.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Graded	5B	Distinct gradient of expression from pigment epithelium to ganglion cell layer. Much lower expression levels in outer undifferentiated ventricular cells than in inner differentiated amacrine cells.
TS27: retina ganglion cell layer	Present	Ubiquitous	5B

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Strong		5C
TS28: retina inner nuclear layer	Strong	Regionally restricted	5C	High expression in the inner third of the ventricular striatum which becomes the inner nuclear layer. Lower levels of expression in inner rods, similar to outer rods on the opposite side of the outer plexiform layer.
TS28: retina outer nuclear layer	Strong	Regionally restricted	5C	Strong expression in occasional large horizontal cells near the outer plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Strong	Regionally restricted	5D	High levels of expression in all cells in the inner nuclear layer, except inner rods on the inner side of the outer plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Strong		5E	Authors state that the pattern of expression is the same as that seen at P day 11.
TS28: retina inner nuclear layer	Strong		5E	Authors state that the pattern of expression is the same as that seen at P day 11.
TS28: retina outer nuclear layer	Present		5E

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Strong		5F
TS28: retina inner nuclear layer	Strong		5F
TS28: retina outer nuclear layer	Present		5F

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Weak	Widespread	6A	Low levels of expression throughout the limb bud.
TS19: handplate	Present	Regionally restricted	6A	Slightly increased expression in presumptive digital regions.

Structure	Level	Pattern	Image	Note
TS20: hand	Very strong	Regionally restricted	6B	Very strong expression in digital regions and apical ectodermal ridge.
TS20: forelimb interdigital region	Weak		6B

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Strong		6C
TS21: hand	Present	Regionally restricted	6C	Little or no expression in apical ectodermal ridge and interdigital zones.
TS21: forelimb interdigital region	Trace		6C	Little or no expression in apical ectodermal ridge and interdigital zones.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit cartilage condensation	Strong	Regionally restricted	6D	Strong expression in precartilage condensations surrounding cartilage plates.
TS22: forelimb interdigital region	Absent		6D

Structure	Level	Pattern	Image	Note
TS22: limb cartilage condensation	Present	Regionally restricted		Expression at the tips of developing long bones in the limbs.

Structure	Level	Pattern	Image	Note
TS22: liver	Present	Regionally restricted	7	Expression in hematopoietic cells, especially in megakaryocytes.
TS22: liver parenchyma	Absent		7

Structure	Level	Pattern	Image	Note
TS28: liver	Absent

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Graded	8A	Gradient of expression with higher expression in the thicker more proximal region of the lung bud than in the thinner distal epithelium.

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Strong		8B
TS26: lung alveolus	Weak		8B
TS26: lung vascular element	Weak		8B

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Strong	Ubiquitous	9A
TS22: intestine muscularis	Strong	Ubiquitous	9A

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Present	Widespread	9B

Structure	Level	Pattern	Image	Note
TS26: intestine epithelium	Present	Regionally restricted	9C	Expression restricted to the base of the villi.

Structure	Level	Pattern	Image	Note
TS28: crypt of lieberkuhn	Present	Regionally restricted	9D	Expression only in intestinal crypts and lower villi.
TS28: intestinal villus	Present	Regionally restricted	9D	Expression only in intestinal crypts and lower villi.

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Strong		10A
TS20: pretubular aggregate	Strong		10A
TS20: ureter	Strong		10A
TS20: mesenchyme of metanephros	Weak		10A	Weak expression in the surrounding loose mesoderm.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Weak	Regionally restricted	10B	Lower levels of expression in metanephric cap tissue, glomeruli, tubules and collecting ducts.
TS24: collecting duct	Weak		10B	Lower levels of expression in metanephric cap tissue, glomeruli, tubules and collecting ducts.
TS24: pelvic urothelial lining	Absent		10B
TS24: renal medullary interstitium	Absent		10B	No expression in the loose mesenchyme of the medulla.