About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:135686 Hirashima M, et al., Lymphatic vessel assembly is impaired in Aspp1-deficient mouse embryos. Dev Biol. 2008 Apr 1;316(1):149-59
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5476531
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31 (2H8 MAB1398Z)
Detection system: Secondary antibody
Assay notes: The AMeX method was used to process the embryos.
Results
Specimen 2B: embryonic day 14.5; Ppp1r13btm1Suda/Ppp1r13b+ (more )
Note: Triple labeled: red - Pecam1; green - Ppp1r13b reporter (assay MGI:5476529); blue - Lyve1 (assay MGI:5476530).
Structure Level Pattern Image Note
TS22: arterial system Present Regionally restricted 2B Expression was detected in endothelial cells.
TS22: blood vessel Present Regionally restricted 2B Expression was detected in endothelial cells.
TS22: venous system Present Regionally restricted 2B Expression was detected in endothelial cells.
TS22: lymphatic system Weak Regionally restricted 2B Expression was detected in endothelial cells.
Specimen 2C: embryonic day 14.5; Ppp1r13btm1Suda/Ppp1r13b+ (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS22: arterial system Present Regionally restricted 2C Expression was detected in endothelial cells.
TS22: blood vessel Present Regionally restricted 2C Expression was detected in endothelial cells.
TS22: lymphatic system Weak Regionally restricted 2C Expression was detected in endothelial cells.
Specimen 3E: embryonic day 14.5 (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 3E Expression was detected in endothelial cells.
TS22: lymphatic system Weak Regionally restricted 3E Expression was detected in endothelial cells.
Specimen 3F: embryonic day 14.5; Ppp1r13btm1Suda/Ppp1r13btm1Suda (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 3F Expression was detected in endothelial cells. Blood vessels appear relatively unaffected in the mutant.
TS22: lymphatic system Weak Regionally restricted 3F Expression was detected in endothelial cells. Lymphatic vessels appear distended.
Specimen 4A/E: embryonic day 14.5; Ppp1r13btm1Suda/Ppp1r13b+ (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 4A , 4E Expression was detected in endothelial cells in back skin.
TS22: lymphatic system Weak Regionally restricted 4A , 4E Expression was detected in endothelial cells in back skin.
Specimen 4B/F: embryonic day 14.5; Ppp1r13btm1Suda/Ppp1r13btm1Suda (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 4B , 4F Blood vessels were relatively unaffected in the mutant.
TS22: lymphatic system Weak Regionally restricted 4B , 4F Lymphatic vessels were disorganized and partly distended in the mutant.
Specimen 4G/K: embryonic day 15.5; Ppp1r13btm1Suda/Ppp1r13b+ (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS23: blood vessel Present Regionally restricted 4G , 4K Expression was detected in endothelial cells in back skin.
TS23: lymphatic system Weak Regionally restricted 4K , 4G Expression was detected in endothelial cells in back skin.
Specimen 4H/L: embryonic day 15.5; Ppp1r13btm1Suda/Ppp1r13btm1Suda (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS23: blood vessel Present Regionally restricted 4L , 4H Blood vessels were relatively unaffected in the mutant.
TS23: lymphatic system Weak Regionally restricted 4H , 4L Collecting vessels were tortuous and distended in the mutant. Isolated ball-like structures of numerous lymphatic endothelial cells - lymphatic islands - were detected in the mutant.
Specimen S1A/E: embryonic day 16.5; Ppp1r13btm1Suda/Ppp1r13b+ (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS24: blood vessel Present Regionally restricted S1A , S1E Expression was detected in endothelial cells in back skin.
TS24: lymphatic system Weak Regionally restricted S1A , S1E Expression was detected in endothelial cells in back skin.
Specimen S1B/F: embryonic day 16.5; Ppp1r13btm1Suda/Ppp1r13btm1Suda (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:5476537).
Structure Level Pattern Image Note
TS24: blood vessel Present Regionally restricted S1F , S1B Blood vessels were relatively unaffected in the mutant.
TS24: lymphatic system Weak Regionally restricted S1F , S1B The lymphatic network was defective and lymphatic islands were detected in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory