Gene Expression Data
Assay Details
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Assay
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Reference:
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J:134562
Trapp ML, et al., Defects in ciliary localization of Nek8 is associated with cystogenesis. Pediatr Nephrol. 2008 Mar;23(3):377-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5475530
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Gene symbol:
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Nek8
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Gene name:
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NIMA (never in mitosis gene a)-related expressed kinase 8
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Antibody:
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anti-Nek8
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Data was shown in Table 2.
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Results
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Specimen
+/jck non-cystic:
postnatal day 7; Nek8jck/Nek8 +
(more  )
Specimen
+/jck non-cystic:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nek8jck/Nek8+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal tubule |
Present |
Regionally restricted |
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Expressed in the luminal cilia. Expressed in 69% medullary dubules, 49% in cortical tubules.
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| TS28: cortical renal tubule |
Present |
Regionally restricted |
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Expressed in the luminal cilia. Expressed in 49% cortical tubules.
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Specimen
jck/jck non-cystic:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nek8jck/Nek8jck
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal tubule |
Present |
Regionally restricted |
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Decreased expression, compared with heterozygous control. Expressed in the luminal cilia. Expressed in 29% medullary dubules, 23% in cortical tubules.
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| TS28: cortical renal tubule |
Present |
Regionally restricted |
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Decreased expression, compared with heterozygous control. Expressed in the luminal cilia. Expressed in 23% cortical tubules.
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Specimen
jck/jck cystic:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nek8jck/Nek8jck
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal tubule |
Present |
Regionally restricted |
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Expressed in the luminal cilia. Expressed in 26% medullary dubules, 26% in cortical tubules. Decreased expression, compared with heterozygous control.
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| TS28: cortical renal tubule |
Present |
Regionally restricted |
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Expressed in the luminal cilia. Expressed in 26% cortical tubules. Decreased expression, compared with heterozygous control.
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Analysis Tools
