Probe:	Gjb1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: inner ear mesenchyme	Present	Regionally restricted	2A	Expression was detected in the mesenchyme surrounding the otocyst.
TS20: inner ear	Present	Regionally restricted	2A	Expression was detected in the subdomains of the otocyst.
TS20: cochlear ganglion	Trace	Regionally restricted	2A	Expression was dispersed and almost undetectable.

Structure	Level	Pattern	Image	Note
TS23: inner ear mesenchyme	Present	Regionally restricted	2B	Expression was detected in the mesenchyme surrounding the otocyst.
TS23: inner ear	Present	Regionally restricted	2B	Expression was detected in subdomains of the otocyst.
TS23: cochlear duct epithelium	Present	Regionally restricted	2B	Expression was detected in the rostral wall of the cochlear duct from which the Reissner's membrane will form.

Structure	Level	Pattern	Image	Note
TS26: cochlea	Present	Regionally restricted	2C	Expression was detected in fibrocytes, basilar membrane cells, and Reissner membrane cells.
TS26: organ of Corti	Present	Regionally restricted	2C	Expression was detected in Hensen and pillar cells.

Structure	Level	Pattern	Image	Note
TS27: spiral limbus	Present	Regionally restricted	2D	Expression was detected in the limbal dark and limbal light cells.
TS27: cochlear ganglion	Present	Non-Uniform	2D	Expression was dispersed and uneven.
TS27: vestibular membrane	Present		2D
TS27: organ of Corti	Strong	Regionally restricted	2D , 2E	Expression was detected in the Hensen and pillar cells.
TS27: basilar membrane	Strong		2D , 2E

Structure	Level	Pattern	Image	Note
TS27: vestibular membrane	Strong		2F
TS27: stria vascularis	Strong	Regionally restricted	2F	Expression was intense in the intermediate and basal cells.
TS27: cochlea	Strong	Regionally restricted	2F	Expression was intense in type II fibrocytes.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Non-Uniform	2G
TS28: vestibular membrane	Present		2G
TS28: internal spiral sulcus	Present	Regionally restricted	2G	Expression was detected in the inner sulcus and in the regressing Kolliker organ.
TS28: organ of Corti	Present	Regionally restricted	2H , 2G	Expression was detected in the outer and inner hair cells, pillar cells and Hensen cells.
TS28: basilar membrane	Present		2G , 2H

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Strong	Regionally restricted	2I	Expression was intense in type II fibrocytes and basal and intermediate cells.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Non-Uniform	2J
TS28: organ of Corti	Ambiguous		2J , 2K	Expression was negative or very weak.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	2L	Expression was detected in type II fibrocytes in the suprastial zone close to the stria vascularis.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	2M	Expression was detected in type II fibrocytes.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Absent		2N
TS28: cochlear ganglion	Absent		2N
TS28: organ of Corti	Absent		2N