Antibody:	Prdm8 antibody 045R2
Detection system:	Alexa Fluor 488
Assay notes:	It is possible that another antibody (MGI:5474232) was used in this assay.

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		2G , 2E	Expression mainly in the inner nuclear layer.
TS26: neural retina	Present	Regionally restricted	2E , 2G	Expression in a small number of cells in the ganglion cell layer.
TS26: retina outer nuclear layer	Absent		2E , 2G	No expression in the outer neuroblast layer.

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		2K , 2I	Expression mainly in the inner nuclear layer.
TS26: neural retina	Present	Regionally restricted	2I , 2K	Expression in a small number of cells in the ganglion cell layer.
TS26: retina outer nuclear layer	Absent		2I , 2K	No expression in the outer neuroblast layer.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	3G , 3E	Expression in innermost layer of inner nuclear layer.
TS27: retina ganglion cell layer	Present		3E , 3G	Expression in a smaller number of cells ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	3K , 3I	Expression in innermost layer of inner nuclear layer. Weak expression in outer portion of inner nuclear layer.
TS27: retina ganglion cell layer	Present		3K , 3I	Smaller number of expressing cells in ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	3M , 3O	Expression in innermost layer of inner nuclear layer.
TS27: retina ganglion cell layer	Present		3O , 3M	Smaller number of expressing cells in ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted		Expression in the outer portion of the inner nuclear.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4E , 4G
TS28: retina ganglion cell layer	Absent		4G , 4E

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4K , 4I
TS28: retina ganglion cell layer	Absent		4I , 4K

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4O , 4M
TS28: retina ganglion cell layer	Absent		4O , 4M

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4S , 4Q
TS28: retina ganglion cell layer	Absent		4Q , 4S

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4W , 4U

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4CC , 4EE
TS28: retina ganglion cell layer	Absent		4EE , 4CC

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	5A	Predominant expression in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	5E	Predominant expression in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present		5H , 5F
TS19: neural tube	Present	Regionally restricted	5H , 5F	Expression in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	5I	Predominant expression in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present		5L , 5J
TS19: neural tube	Present	Regionally restricted	5J , 5L	Expression restricted to ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6A	Expression restricted to ventral spinal cord.
TS21: spinal cord marginal layer	Present	Scattered	6A	Scattered pattern of expression in the marginal zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6E	Predominant expression in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present		6H , 6F
TS21: spinal cord	Present	Regionally restricted	6F , 6H	Expression in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6I	Predominant expression in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present		6J , 6L
TS21: spinal cord	Present	Regionally restricted	6L , 6J	Expression in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6Q	Predominant expression in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present		6T , 6R
TS21: spinal cord	Strong	Regionally restricted	6T , 6R	Expression in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Strong		7Z , 7X	Strongest expression in layer 4.