Opa3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:181412 Powell KA, et al., Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III. Invest Ophthalmol Vis Sci. 2011 Jun;52(7):4369-80
Assay type:	RT-PCR
MGI Accession ID:	MGI:5471333
Gene symbol:	Opa3
Gene name:	optic atrophy 3

Probe:	F1 qPCR, R3 qPCR
Assay notes:	Quantitative RT-PCR. Relative expression levels normalized to 18S RNA.

Results

Image: 1C transcript b

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
2-P7 +/+	P7	TS28: lens	Present	1 µg; total RNA	Not Specified		Not Specified
2-P7 -/-	P7	TS28: lens	Present	1 µg; total RNA	involves: C3H * C57BL/6JCrl	Opa3^m1Votr/Opa3^m1Votr	Not Specified
2-P30 +/+	P30	TS28: lens	Present	1 µg; total RNA	Not Specified		Not Specified
2-P30 -/-	P30	TS28: lens	Present	1 µg; total RNA	involves: C3H * C57BL/6JCrl	Opa3^m1Votr/Opa3^m1Votr	Not Specified

Notes:
(a) In a quantitative assessment of the relative expression of all isoforms (see MGI:5471331) compared to isoform b only (this assay), isoform b was expressed at less than 10-fold levels. The presence of the mutation did not disturb the ratio.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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