Probe:	HRF probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Ubiquitous
TS11: yolk sac	Present	Ubiquitous

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Ubiquitous	4B/C
TS13: yolk sac	Present	Ubiquitous	4B/C

Structure	Level	Pattern	Image	Note
TS28: uterus	Strong	Regionally restricted	4B/C	Strong expression in smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present		4D
TS19: cardinal vein	Weak		4D
TS19: foregut epithelium	Absent		4D

Structure	Level	Pattern	Image	Note
TS19: cardiovascular system	Present	Regionally restricted	4E	Expression in endothelial cells of capillaries of the perineural plexus.
TS19: brain	Weak	Regionally restricted	4E	Authors report weak expression in neuroectodermal epithelium of brain anlage and spinal cord.
TS19: future spinal cord	Weak	Regionally restricted	4E	Authors report weak expression in neuroectodermal epithelium of brain anlage and spinal cord.
TS19: head mesenchyme	Absent		4E

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present
TS19: somite	Present

Structure	Level	Pattern	Image	Note
TS23: brain vascular element	Strong	Regionally restricted	4F	Strong expression in endothelial cells of brain capillaries.
TS23: 4th ventricle choroid plexus	Strong		4F

Structure	Level	Pattern	Image	Note
TS23: brain vascular element	Present	Regionally restricted	4G	Expression in endothelial cells of brain capillaries.

Structure	Level	Pattern	Image	Note
TS23: 4th ventricle choroid plexus	Strong	Regionally restricted	4H	Strong expression in both surface epithelium and core endothelium.

Structure	Level	Pattern	Image	Note
TS23: maturing glomerular tuft	Strong		4I
TS23: visceral epithelium of maturing renal corpuscle	Present	Regionally restricted	4I	Expression in podocytes.
TS23: renal small blood vessel	Present	Regionally restricted	4I	Expression in capillaries.
TS23: Bowman's capsule of maturing renal corpuscle	Absent		4I

Structure	Level	Pattern	Image	Note
TS23: skin	Strong	Regionally restricted	4K	Strong expression in brown adipose tissue of neck subcutis localized to fat lobules.

Structure	Level	Pattern	Image	Note
TS23: nasal cavity epithelium	Strong		4L

Structure	Level	Pattern	Image	Note
TS23: lobar bronchus epithelium	Strong		4M
TS23: lung parenchyma	Strong		4M
TS23: lung vascular element	Strong	Regionally restricted	4M	Strong expression in endothelium of large blood vessels.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present
TS23: liver parenchyma	Present
TS23: musculature	Present
TS23: thyroid gland	Present

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	5A/B	Expression in neurons.
TS28: brain blood vessel	Present	Regionally restricted	5A/B	Expression in brain capillaries.

Structure	Level	Pattern	Image	Note
TS28: spleen white pulp	Strong	Regionally restricted	5C	Most prominent expression restricted to central arteries of the white pulp.

Structure	Level	Pattern	Image	Note
TS28: bronchus epithelium	Strong		5D	Most prominent expression in the respiratory epithelium of the bronchi.
TS28: lung parenchyma	Strong		5D
TS28: lung vascular element	Weak	Regionally restricted	5D	Weak expression in endothelium of large vessels.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	5E	Expression in the epithelium of tubular structures.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Ubiquitous	5F (upper panel)

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present	Diffuse		No distinct expression in the glomeruli.
TS28: heart	Present	Diffuse		No distinct expression in the endothelium.
TS28: skeletal muscle	Present	Diffuse		No distinct expression in the endothelium.