Probe:	Irx2 cDNA4
Probe preparation:	Antisense RNA
Assay notes:	At least two different probes between 0.5-1.0kb were used for analysis. The authors did not provide the details of which probe(s) were used to obtain these data but did report that the detected patterns were identical.

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent
TS9: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		3B
TS11: extraembryonic component	Absent		3B

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Present		3H	Expression is present in pre-rhombomere 4.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 4	Present		3K

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong	Non-Uniform	4B	The rostral limit of expression is at the level of the pretectum. There is strong expression in the tectum.
TS15: rhombomere 1	Present		4B
TS15: rhombomere 2	Present		4B
TS15: rhombomere 3	Present		4B
TS15: rhombomere 4	Present		4B
TS15: rhombomere 7	Present		4B
TS15: rhombomere 8	Present		4B
TS15: 1st branchial arch ectoderm	Present		4B
TS15: 2nd branchial arch ectoderm	Present		4B
TS15: foregut	Present		4B
TS15: neural tube	Present		4B

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	4E	The rostral limit of expression is in the dorsalthalamic regions (the prospective domain of the zona limitans intrathalamica).
TS17: head mesenchyme	Present	Regionally restricted	4E	Expression is present in the mesenchyme around the optic vesicle.
TS17: limb	Weak	Regionally restricted	4E	Expression is present in the dorsal portion.
TS17: hindbrain	Present		4E
TS17: midbrain	Present		4E
TS17: somite	Absent		4E

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		5B
TS15: surface ectoderm	Present		5B
TS15: neural tube	Present		5B

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		5E
TS15: surface ectoderm	Present		5E

Structure	Level	Pattern	Image	Note
TS15: tracheal diverticulum	Present		5H
TS15: neural tube lateral wall	Weak	Regionally restricted	5H	Expression is confined to the alar plate along the entire spinal cord.
TS15: surface ectoderm	Present		5H

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present		5K
TS15: neural tube lateral wall	Weak	Regionally restricted	5K	Expression is confined to the alar plate along the entire spinal cord.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		6B
TS15: otocyst	Present	Regionally restricted	6B	Expression is present in lateroventral region; there is no expression in the dorsal region.
TS15: head mesenchyme	Present	Regionally restricted	6B	Expression is present on the ventral side of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		6E
TS15: otocyst	Present	Regionally restricted	6E	Expression is present in lateroventral region; there is no expression in the dorsal region.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Widespread		Expression is present in the entire otic vesicle.