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Gene Expression Data
Assay Details
Assay
Reference: J:29932 Fedtsova NG, et al., Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors. Mech Dev. 1995 Nov;53(3):291-304
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5468310
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-160kDa neurofilament
Detection system: fluorescein
Assay notes: Specimens were fixed in 60% ethanol and 4% formaldehyde.
Results
Specimen 6a/b: embryonic day 10.0 (more )
Note: 27 somites. Double labeled: red - Nefm; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 6a , 6b Expression in the caudal midbrain surrounding Brn-3.0-expressing neurons of the mesencephalic nucleus of the trigeminal (MesV) at the isthmus.
TS15: future hindbrain Present Regionally restricted 6b , 6a Expression in a few fibers just lateral to Brn-3.0-expressing cells in the ventral pons.
Specimen 6c: embryonic day 10.0 (more )
Note: 27 somites. Double labeled: red - Nefm; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS15: dorsal root ganglion Present Regionally restricted 6c Expression only detectable in cells positive for Brn-3.0.
Specimen 6d: embryonic day 10.5 (more )
Note: 40 somites. Double labeled: red - Nefm; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS18: dorsal root ganglion Present Regionally restricted 6d
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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