Probe:	Bmp4 cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	1C	Expression is concentrated toward mesial tip of the tooth germ (epithelial thickening).
TS20: jaw primordium mesenchyme	Present	Regionally restricted	1C	Expressed in the mesenchyme underlying the tooth germ, with a strong mesial bias.

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Absent		1F	As the epithelial placode involutes into the underlying jaw mesenchyme, no expression in the epithelium.
TS20: jaw primordium mesenchyme	Present	Regionally restricted	1F	As the epithelial placode involutes into the underlying jaw mesenchyme, expression continues to be in mesial mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS22: enamel knot	Present		1I
TS22: dental papilla	Present	Widespread	1I	Expressed throughout the dental papilla, with highest levels of expression adjacent to epithelium.

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Present	Regionally restricted	1L	Expression is primarily restricted to papilla adjacent to epithelium, also concentrated at mesial aspects of cusps.

Structure	Level	Pattern	Image	Note
TS24: enamel organ	Present	Regionally restricted	1O	Expressed in the differentiating ameloblasts.
TS24: dental papilla	Present	Regionally restricted	1O	Expressed in the odontoblasts.

Structure	Level	Pattern	Image	Note
TS21: vibrissa epidermal component	Present		2C	Expressed in mesenchyme underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS22: vibrissa epidermal component	Present	Regionally restricted	2F	Expressed in mesenchyme underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS24: vibrissa follicle	Present	Regionally restricted	2I	Expressed in the inner root sheath and precortical cells.
TS24: vibrissa dermal component	Present	Regionally restricted	2I	Expressed in dermal papilla.
TS24: vibrissa inner root sheath	Present		2I

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present	Regionally restricted	3B	Expressed in mesenchyme underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present	Regionally restricted	3D	Expressed in mesenchyme underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS19: palatal shelf epithelium	Present	Regionally restricted	4B	Expressed in mesial epithelium.
TS19: palatal shelf mesenchyme	Present		4B

Structure	Level	Pattern	Image	Note
TS22: palatal rugae epithelium	Present	Regionally restricted	4D	Most strongly expressed in a region slightly mesial to that of Shh.
TS22: palatal rugae mesenchyme	Present	Regionally restricted	4D	Expressed in mesenchyme underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS19: main bronchus epithelium	Present	Regionally restricted	5C	Expressed in the ventral bronchial epithelium.
TS19: stomach mesenchyme	Present	Regionally restricted	5C	Expressed in mesenchyme surrounding forestomach.
TS19: midgut	Present	Regionally restricted	5C	Expressed in mesenchyme surrounding midgut. Expressed in a thin layer of mesenchyme directly beneath the epithelium.
TS19: main bronchus mesenchyme	Present	Regionally restricted	5C	Expressed in mesenchyme surrounding bronchi.

Structure	Level	Pattern	Image	Note
TS21: stomach mesenchyme	Present	Widespread	5F

Structure	Level	Pattern	Image	Note
TS20: hindgut mesentery	Present	Regionally restricted	5I	Expressed in mesenchyme surrounding the epithelium.
TS20: midgut mesenchyme	Present	Regionally restricted	5I	Expressed in mesenchyme surrounding the epithelium.
TS20: caudal urogenital sinus mesenchyme	Present	Regionally restricted	5I	Expressed in mesenchyme surrounding the epithelium of urethra.

Structure	Level	Pattern	Image	Note
TS20: midgut mesenchyme	Present	Regionally restricted	5L	Expressed in mesenchyme surrounding the epithelium.

Structure	Level	Pattern	Image	Note
TS22: duodenum	Present	Regionally restricted	5P	Expressed in mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: rectum mesenchyme	Present		5S

Structure	Level	Pattern	Image	Note
TS19: urogenital sinus	Present	Regionally restricted	6B	Expressed in mesenchyme surrounding the epithelium.

Structure	Level	Pattern	Image	Note
TS22: male reproductive system	Present	Regionally restricted	6D	Expressed in mesenchyme surrounding the epithelium of vas deferens.
TS22: bladder	Present	Regionally restricted	6D	Expressed in mesenchyme surrounding the epithelium.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular canal	Present	Regionally restricted	6H	Expressed in myocardium underlying the atrioventricular valve.
TS20: outflow tract	Present	Regionally restricted	6H	Expressed in myocardium underlying the truncus arteriosus.

Structure	Level	Pattern	Image	Note
TS22: lobar bronchus epithelium	Present	Regionally restricted	7B	Expressed in a polarized fashion within bronchioles, apparently corresponding to branch points.

Structure	Level	Pattern	Image	Note
TS19: trachea mesenchyme	Present
TS19: lung mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS22: foregut mesenchyme	Present	Regionally restricted		Expressed in mesenchyme surrounding the epithelium.
TS22: bronchus mesenchyme	Present
TS22: trachea mesenchyme	Present
TS22: lung mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS24: hard palate palatal rugae epithelium	Present
TS24: hard palate palatal rugae mesenchyme	Present