Probe:	Ebk probe 7
Probe preparation:	labelled with S35 DNA
Visualized with:	Autoradiography
Assay notes:	One of several probes (MGI:5467967, MGI:5467974, MGI:5467970) may have been used. Authors state that different probes gave the same results.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present
TS28: cerebellum	Absent
TS28: cerebral cortex	Absent
TS28: thalamus	Absent
TS28: hypothalamus	Absent			No expression in the mammillary area.
TS28: tectum	Absent			No expression in the anterior tectum.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present
TS27: cochlear duct	Present
TS27: hippocampus	Present
TS27: thalamus	Present
TS27: trigeminal ganglion	Present
TS27: tectum	Present	Regionally restricted		Expression in the anterior tectum.
TS27: hypothalamus	Present	Regionally restricted		Expression in the mamillary area.
TS27: cerebellum	Present	Regionally restricted
TS27: hindbrain	Present	Regionally restricted
TS27: nose	Present	Regionally restricted		Expression mainly in mesoderm deep to the epithelium.
TS27: oral region	Present	Regionally restricted		Expression mainly in mesoderm deep to the epithelium.
TS27: cartilage	Present	Regionally restricted
TS27: meninges	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present
TS25: cochlear duct	Present
TS25: thalamus	Present
TS25: trigeminal ganglion	Present
TS25: hypothalamus	Present	Regionally restricted		Expression in the mammillary area.
TS25: tectum	Present	Regionally restricted		Expression in the anterior tectum.
TS25: cerebellum	Present	Regionally restricted
TS25: hindbrain	Present	Regionally restricted
TS25: nose	Present	Regionally restricted		Expression mainly in mesoderm deep to the epithelium.
TS25: oral region	Present	Regionally restricted		Expression mainly in mesoderm deep to the epithelium.
TS25: hippocampus	Weak

Structure	Level	Pattern	Image	Note
TS23: brain	Very strong	Regionally restricted		Expression in the subcommissural organ.
TS23: cochlear duct	Strong
TS23: nose	Strong	Regionally restricted		Expression mainly in mesoderm deep to the epithelium
TS23: oral region	Strong	Regionally restricted		Expression mainly in mesoderm deep to the epithelium
TS23: cerebellum	Present
TS23: hippocampus	Present
TS23: meninges	Present
TS23: thalamus	Present
TS23: trigeminal ganglion	Present
TS23: hypothalamus	Present	Regionally restricted		Expression in the mammillary area.
TS23: head	Present	Regionally restricted		Expression at the mesencephalic flexure.
TS23: tectum	Present	Regionally restricted		Expression in the anterior tectum.
TS23: eyelid mesenchyme	Present
TS23: cartilage	Weak
TS23: cerebral cortex	Weak

Structure	Level	Pattern	Image	Note
TS22: bronchus	Present	Regionally restricted
TS22: lung	Present	Regionally restricted
TS22: metanephros	Present	Regionally restricted
TS22: genital tubercle	Present	Regionally restricted		Expression in the dorsal part.
TS22: paraxial mesenchyme	Weak	Regionally restricted		Expression in undifferentiated mesoderm flanking the spinal cord and dorsal root ganglia, possibly including somitic mesoderm.
TS22: heart	Weak	Regionally restricted
TS22: hindlimb digit mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS21: paraxial mesenchyme	Strong	Regionally restricted		Expression in undifferentiated mesoderm flanking the spinal cord and dorsal root ganglia, possibly including somitic mesoderm.
TS21: bronchus	Present
TS21: heart	Present
TS21: lung	Present
TS21: metanephros	Present	Regionally restricted
TS21: genital tubercle	Present	Regionally restricted		Expression in the dorsal part
TS21: gut epithelium	Weak	Regionally restricted

Structure	Level	Pattern	Image	Note
TS20: paraxial mesenchyme	Strong	Regionally restricted		Expression in undifferentiated mesoderm flanking the spinal cord and dorsal root ganglia, possibly including somitic mesoderm.
TS20: bronchus	Present
TS20: heart	Present
TS20: lung	Present
TS20: metanephros	Present	Regionally restricted
TS20: genital tubercle	Present	Regionally restricted		Expression in the dorsal part
TS20: gut epithelium	Weak	Regionally restricted

Structure	Level	Pattern	Image	Note
TS19: head	Strong	Regionally restricted		Expression at the mesencephalic flexure.
TS19: lung	Strong
TS19: paraxial mesenchyme	Strong	Regionally restricted		Expression in undifferentiated tissue flanking spinal cord and dorsal root ganglia, including some somitic and marginal mesoderm. Also intense expression around mesonephric duct and on ventral side of aorta.
TS19: 1st branchial arch	Present
TS19: hindbrain	Present	Regionally restricted		Expression in rhombomeres.
TS19: forebrain	Present	Regionally restricted		Expression only at midline.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Very strong	Regionally restricted		Expression in dorsal forebrain/midbrain.
TS17: midbrain	Very strong	Regionally restricted		Expression in dorsal forebrain/midbrain.
TS17: head	Strong	Regionally restricted		Expression at the mesencephalic flexure
TS17: lung	Strong
TS17: otocyst	Strong
TS17: heart	Strong	Regionally restricted
TS17: paraxial mesenchyme	Strong	Regionally restricted		Expression in undifferentiated tissue flanking spinal cord and dorsal root ganglia, including some somitic and marginal mesoderm. Also intense expression around mesonephric duct and on ventral side of aorta.
TS17: hindbrain	Present	Regionally restricted		Expression in the medulla.
TS17: 1st branchial arch	Present

Structure	Level	Pattern	Image	Note
TS15: otocyst	Strong
TS15: future hindbrain	Strong	Regionally restricted		Expression in the medulla.
TS15: paraxial mesenchyme	Strong	Regionally restricted		Expression in undifferentiated tissue flanking spinal cord and dorsal root ganglia, including some somitic and marginal mesoderm. Also intense expression around mesonephric duct and on ventral side of aorta.
TS15: future forebrain	Present	Regionally restricted		Expression in the dorsal forebrain/midbrain.
TS15: future midbrain	Present	Regionally restricted		Expression in the dorsal forebrain/midbrain.
TS15: heart	Weak	Regionally restricted
TS15: 1st branchial arch	Absent
TS15: lung	Absent