Probe:	TSC-22 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		1B
TS9: extraembryonic component	Present		1B

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	1B inset	Expression in condensing mesenchyme of the hair follicle.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Strong
TS9: extraembryonic visceral endoderm	Weak

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Widespread	1C
TS11: allantois	Present		1C
TS11: amnion	Present		1C
TS11: yolk sac	Present		1C

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS13: future midbrain neural fold	Present		1D
TS13: future prosencephalon neural fold	Present		1D
TS13: future hindbrain neural fold	Present	Regionally restricted	1D	No expression in the myelencephalon.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural fold	Present
TS13: somite	Present

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Strong		1E
TS15: eye	Strong		1E
TS15: otocyst	Strong		1E
TS15: limb mesenchyme	Present	Uniform	1E
TS15: central nervous system	Present	Widespread	1E
TS15: heart	Present	Widespread	1E

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	1F	Expression restrcted to outer portion of optic vesicle, in direct contact with surface epithelium, or lens placode.
TS15: head mesenchyme	Present	Regionally restricted	1F	Expression in head mesenchyme cells that are in close proximity to cephalic vesicles.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Weak		1G
TS15: head mesenchyme	Present		1G

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	1H	Expression in head mesenchyme cells that are in close proximity to cephalic vesicles.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	1I	Expression elevated in the progress zone.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present	Regionally restricted	1J	Expression elevated in the progress zone.

Structure	Level	Pattern	Image	Note
TS22: limb mesenchyme	Present	Regionally restricted	1K	Elevated expression in joint forming regions.
TS22: skin	Present	Regionally restricted	1K	Dotted pattern of expression in the skin.

Structure	Level	Pattern	Image	Note
TS23: limb mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Widespread	2A
TS21: alar columns	Strong		2A	Stronger expression in the alar region.
TS21: dorsal root ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS21: notochord	Present		2B
TS21: gut epithelium	Present	Regionally restricted	2B	Expression in many areas of the gut epithelium including the hindgut.
TS21: hindgut	Present	Regionally restricted	2B	Expression in many areas of the gut epithelium including the hindgut.
TS21: pancreas	Present		2B

Structure	Level	Pattern	Image	Note
TS21: esophagus	Present
TS21: lung	Present
TS21: stomach	Present

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present	Regionally restricted	2C	Expression in many areas of the gut epithelium including the hindgut.

Structure	Level	Pattern	Image	Note
TS21: ovary	Present		2D
TS21: paramesonephric duct of female	Present		2D

Structure	Level	Pattern	Image	Note
TS21: femur cartilage condensation	Present		2E	Expression in cartilage primordia throughout the body, particularly in the developing limb.
TS21: metatarsus pre-cartilage condensation	Present		2E	Expression in cartilage primordia throughout the body, particularly in the developing limb.

Structure	Level	Pattern	Image	Note
TS25: hindlimb mesenchyme	Present	Regionally restricted	2F	Expression in cartilage primordia throughout the body, particularly in the developing limb.

Structure	Level	Pattern	Image	Note
TS25: cartilage	Present	Widespread		Expression in cartilage primordia throughout the body, including facial cartilage and bone.

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Present		2G

Structure	Level	Pattern	Image	Note
TS25: facial ganglion	Present		2H
TS25: tooth	Present		2H

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Present		2I

Structure	Level	Pattern	Image	Note
TS25: epidermis	Present		2K
TS25: vibrissa	Present	Regionally restricted	2K	Expression in the condensing mesenchyme of the whisker follicle.
TS25: hair follicle	Absent		2K	No expression in invaginating ectoderm of the hair bud.