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Gene Expression Data
Assay Details
Assay
Reference: J:43464 Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5467545
Gene symbol: En1
Gene name: engrailed 1
Antibody: alpha Enhb-1
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2a: embryonic day 10.5 (more )
Note: Double labeled: red - En1; green - Tubb3 (assay MGI:5467547)
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 2a In the posterior spinal cord, expression was detected in postmitotic neurons migrating out of the ventricular zone. Cells were located at the lateral margins of the ventricular zone and in a few cells close to the lumenal surface.
Specimen 5a & 5c: embryonic day 9.5 (more )
Note: Double labeled: red - En1; green - Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 5a , 5c Expression was detected in a ventral subset of Lim1/2-positive interneurons in the intermediate region.
Specimen 5d: embryonic day 11.5 (more )
Note: Double labeled: red - En1; green - Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 5d Expression was detected in interneurons. Co-expression was noted with Lim1/2.
TS19: sclerotome Present Regionally restricted 5d
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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