Probe:	pB240
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The authors did in situs with several probes, but this one had the lowest background level.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent
TS11: trophectoderm	Present			Expression is present in the trophoblastic tissue.

Structure	Level	Pattern	Image	Note
TS13: embryo	Strong	Regionally restricted	4B , 4A	Expression is present in the anterior portion of the embryo.
TS13: trophectoderm	Present		4A

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Strong	Regionally restricted	4C , 4D	Expression is present in the lateral head mesenchyme; there is little or no expression in the sparse mesenchyme medial to the brain plate.
TS13: head mesenchyme derived from neural crest	Present		4C , 4D
TS13: future spinal cord neural fold	Present		4C , 4D
TS13: embryo ectoderm	Strong		4D , 4C	Expression is present in the surface ectoderm.
TS13: future brain	Absent		4D , 4C	There is little or no expression in the brain plate.
TS13: yolk sac	Absent		4C

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Present		4E
TS13: future spinal cord neural fold	Present		4E	Expression is present in the neural folds but not in the neural plate.
TS13: somite	Absent		4E
TS13: trunk paraxial mesenchyme	Absent		4E

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present		5A
TS15: branchial arch mesenchyme derived from neural crest	Present		5A
TS15: head mesenchyme derived from neural crest	Present		5A
TS15: facio-acoustic preganglion complex	Present		5A
TS15: trigeminal preganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS15: mesonephros	Weak			Expression is present in mesonephric regions.
TS15: dorsal aorta	Present	Diffuse
TS15: gut	Present	Diffuse
TS15: trunk	Present	Regionally restricted		Expression is present in the lateral body.
TS15: limb mesenchyme	Present	Diffuse

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		5D
TS17: hindbrain	Present	Regionally restricted	5D	Expression is diffusely present in the lateral neuroepithelium in the central nervous sytem.
TS17: neural tube	Present	Regionally restricted	5D	Expression is diffusely present in the lateral neuroepithelium in the central nervous sytem.
TS17: somite	Absent		5D

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Strong		5F
TS17: trigeminal ganglion	Strong		5F
TS17: 1st branchial arch mandibular component mesenchyme	Strong	Regionally restricted	5F	There is strong expression in regions of facial mesenchyme heavily populated by neural crest cells.
TS17: 1st branchial arch maxillary component mesenchyme	Strong	Regionally restricted	5F	There is strong expression in regions of facial mesenchyme heavily populated by neural crest cells.
TS17: head mesenchyme derived from neural crest	Strong	Regionally restricted	5F	There is strong expression in regions of facial mesenchyme heavily populated by neural crest cells.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		5G
TS17: future spinal cord	Present	Regionally restricted	5G	Expression is present in lateral regions of the spinal cord, adjacent to the dorsal root ganglion.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		6A
TS19: hindbrain	Strong	Regionally restricted	6A	Expression is present in the lateral central nervous system. The expression extends anteriorly as far as the hindbrain-midbrain junction.
TS19: myelencephalon lateral wall	Strong	Regionally restricted	6A	Expression is present in the lateral central nervous system. The expression extends anteriorly as far as the hindbrain-midbrain junction.
TS19: neural tube	Strong	Regionally restricted	6A	Expression is present in the lateral central nervous system. The expression extends anteriorly as far as the hindbrain-midbrain junction.
TS19: fronto-nasal process	Present		6A

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		6B
TS19: hindbrain	Present	Regionally restricted	6B	Expression is present in the lateral central nervous system.
TS19: neural tube	Present	Regionally restricted	6B	Expression is present in the lateral central nervous system.
TS19: sympathetic ganglion	Present		6B
TS19: acoustic ganglion	Present		6B
TS19: facial ganglion	Present		6B
TS19: inferior glossopharyngeal ganglion	Weak	Diffuse	6B	Low, diffuse expression is seen in the petrosal ganglia.
TS19: inferior vagus ganglion	Weak	Diffuse	6B	Low, diffuse expression is seen in the nodose ganglia.
TS19: superior glossopharyngeal ganglion	Weak	Diffuse	6B	Low, diffuse expression is seen in the superior ganglia.
TS19: spinal nerve	Weak	Diffuse	6B	Low, diffuse expression is seen in the spinal nerve paths.
TS19: cranial nerve	Weak	Diffuse	6B	Low, diffuse expression is seen in the cranial nerve paths.
TS19: heart	Absent		6B

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	6C	Expression is mediolateral and mainly dorsal to the sulcus limitans.
TS19: myelencephalon	Present	Regionally restricted	6C	Expression is mediolateral and mainly dorsal to the sulcus limitans.
TS19: acoustic ganglion	Present		6C	Expression is present in the ganglia and their boundary caps.
TS19: facial ganglion	Present		6C	Expression is present in the ganglia and their boundary caps.
TS19: pharynx mesenchyme	Present		6C	Expressionis present in the facial mesenchyme lateral to the pharynx.
TS19: surface ectoderm	Present		6C	Expression is present in the epidermis.
TS19: future spinal cord	Present	Regionally restricted	6C	Expression is at least to the level of the hindlimbs.
TS19: dorsal root ganglion	Present		6C
TS19: mesonephros	Present		6C	Expression is present in mesonephric regions.
TS19: forelimb bud	Present		6C
TS19: heart	Absent		6C

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present		6D	Expression is present in facial mesenchyme.
TS19: surface ectoderm	Present		6D	Expression is present in epidermis.
TS19: dorsal root ganglion	Present		6D
TS19: future spinal cord	Present		6D
TS19: nephric duct, mesonephric portion	Present		6D	Expression is present in the mesonephric duct.
TS19: diencephalon	Present		6D

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Strong		7A/B	There is strong expression in the facial mesenchyme adjacent to the nasal epithelia and along the nasal lacrimal groove.
TS20: epidermis	Present		7A/B
TS20: trigeminal ganglion	Present		7A/B
TS20: metencephalon lateral wall	Present	Regionally restricted	7A/B	Expression is present in the lateral anterior metencephalon.
TS20: cornea	Present		7A/B

Structure	Level	Pattern	Image	Note
TS20: frontal process mesenchyme	Strong		7C/D	There is strong expression in the facial mesenchyme adjacent to the nasal epithelia and along the nasal lacrimal groove.
TS20: hand mesenchyme	Present	Regionally restricted	7C/D	Expression is present in the progress zone.
TS20: forelimb mesenchyme	Present	Regionally restricted	7C/D	Expression is present in the progress zone and small medial patch.
TS20: forelimb epithelium	Present		7C/D

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata alar plate	Present		7E/F	Areas of expression include the sulcus limitans.
TS20: spinal cord	Present		7E/F