Probe:	pmcRet7
Probe preparation:	Antisense RNA
Assay notes:	Embryos were derived from crosses between FVB/N inbred mice, or between (FVB/N x C57BL/6)F1, or (C57BL10 x CBA)F1 hybrid mice.

Structure	Level	Pattern	Image	Note
TS11: trophectoderm	Present
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	3A/B	Expression is in a group of neural crest cells emerging from rhombomere 4.
TS14: surface ectoderm	Present	Regionally restricted	3A/B	Expression is in a domain of surface ectoderm extending posterior to the positive neural crest cells.
TS14: future brain	Absent		3A/B

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	3C/D	Expression is restricted to a group of neural crest cells immediately anterior to the otic vesicle, and extending from the dorsal edge of the embryo into the forming second branchial arch.
TS14: 2nd branchial arch	Present	Regionally restricted	3C/D

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		3E/F
TS15: branchial arch ectoderm	Present	Regionally restricted	3E/F	Expression in the 4th branchial arch.
TS15: branchial arch endoderm	Present	Regionally restricted	3E/F	Expression in the 4th branchial arch.
TS15: branchial arch mesenchyme	Strong	Regionally restricted	3E/F	Expression in the 4th branchial arch.
TS15: 3rd branchial arch endoderm	Strong		3E/F

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	3G/H	Expression is at the posterior edge.
TS15: otocyst mesenchyme	Present	Regionally restricted	3G/H	Expression is at the posterior edge.
TS15: branchial arch ectoderm	Present		3G/H
TS15: branchial arch endoderm	Present		3G/H

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Strong	Regionally restricted	4A	Expression is in neural crest cells emerging from rhombomere 4.
TS13: surface ectoderm	Present	Regionally restricted	4A	Expression is in a well-defined domain of surface ectoderm located ventrocaudally to the expression of rhombomere 4 neural crest.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		4B
TS15: branchial arch	Present	Regionally restricted	4B	Expression is in the posterior branchial arches.
TS15: 2nd branchial arch	Absent		4B
TS15: glossopharyngeal-vagus preganglion complex	Absent		4B
TS15: trigeminal preganglion	Absent		4B

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Present		4C
TS17: glossopharyngeal ganglion	Present	Regionally restricted	4C	Inferior complex, absent in superior complex.
TS17: vagus ganglion	Present	Regionally restricted	4C	Inferior complex, absent in superior complex.
TS17: trigeminal ganglion	Absent

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present		5A/B

Structure	Level	Pattern	Image	Note
TS21: facial ganglion	Present
TS21: inferior glossopharyngeal ganglion	Present
TS21: inferior vagus ganglion	Present
TS21: superior glossopharyngeal ganglion	Present
TS21: superior vagus ganglion	Present
TS21: trigeminal ganglion	Present
TS21: vestibulo-cochlear ganglion	Present

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present
TS22: inferior glossopharyngeal ganglion	Present
TS22: inferior vagus ganglion	Present
TS22: superior glossopharyngeal ganglion	Present
TS22: superior vagus ganglion	Present
TS22: vestibulo-cochlear ganglion	Present

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Very strong	Punctate	5C	Expression is in a subset of cells.
TS21: spinal nerve	Absent		5C

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present	Regionally restricted		Dorsal expression between the neural tube and the surface ectoderm and ventrally in the sclerotome, areas of the somites in which the trunk neural crest cells first migrate and subsequently condense to form the dorsal root ganglia.
TS15: sclerotome	Present	Regionally restricted		Ventral expression.
TS15: migrating neural crest	Present	Regionally restricted
TS15: neural tube lateral wall	Present	Regionally restricted		Ventrolateral expression.

Structure	Level	Pattern	Image	Note
TS16: trunk mesenchyme	Strong	Single cells	6A	Expression is in single mesenchymal cells migrating from the posterior branchial arches towards the foregut.
TS16: peripheral nervous system	Strong	Regionally restricted	6A	Expression is in the sympathetic ganglia forming on either side of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS16: midgut	Present	Single cells	6B	Expression is in migrating cells in a rostrocaudal direction within the mesenchyme of the midgut wall.

Structure	Level	Pattern	Image	Note
TS19: intestine	Present	Single cells	6C	Expression is in cells that have migrated into the distal portion of the developing small intestine, and are starting to coalesce to form the enteric ganglia.

Structure	Level	Pattern	Image	Note
TS22: intestine	Strong	Regionally restricted	6D	Expression is in a ring-like shape in the myenteric ganglia of the stomach and small intestine.
TS22: stomach	Strong	Regionally restricted	6D	Expression is in a ring-like shape in the myenteric ganglia of the stomach and small intestine.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	7A/B	Expression is ventral.
TS14: trunk	Present	Regionally restricted	7A/B	Expression is lateral to the somite in the nephrotome.

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present		7C/D
TS21: dorsal root ganglion	Present	Single cells	7C/D

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present	Regionally restricted	8A/B	Expression is at the innermost layer of the neuroretina.

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	8C/D	Expression is in the amacrine and horizontal cell layers, absent in bipolar layer in this albino mouse.
TS28: retina ganglion cell layer	Present		8C/D	Albino mouse.
TS28: photoreceptor layer	Absent		8C/D	Albino mouse.

Structure	Level	Pattern	Image	Note
TS15: nephric duct	Present	Regionally restricted	9A	Highest levels were seen in its caudal part.
TS15: mesonephros	Present		9A

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Strong		9B
TS19: metanephric mesenchyme	Absent		9B

Structure	Level	Pattern	Image	Note
TS25: metanephros	Present	Regionally restricted	9C	Expression is in the outer zone.

Structure	Level	Pattern	Image	Note
TS25: collecting duct	Present		9D
TS25: mature nephron	Absent		9D
TS25: nephron	Absent		9D