Antibody:	clone ECCD-2 (#13-1900)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Widespread	5b	Expression is presentin all embryonic and extraembryonic cells except for parietal endoderm and mesoderm which appeared after gastruation.
TS9: extraembryonic component	Present	Widespread	5b	Expression is presentin all embryonic and extraembryonic cells except for parietal endoderm and mesoderm which appeared after gastruation.
TS9: embryo mesoderm	Absent		5b
TS9: parietal endoderm	Absent		5b

Structure	Level	Pattern	Image	Note
TS28: decidua	Absent		5b
TS28: uterus	Present	Regionally restricted	5b	Expression is present in the epithelium.

Structure	Level	Pattern	Image	Note
TS28: placenta	Present	Regionally restricted	5d	Expression is present in the labyrinth but not in the spongiotrophoblast.
TS28: decidua	Absent		5d

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Widespread	6b	Expression is present in most tissues.
TS12: embryo endoderm	Present	Widespread	6b	Expression is present in most tissues.
TS12: embryo mesenchyme	Absent		6b
TS12: future spinal cord neural plate	Absent		6b

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present		7b
TS16: optic cup	Absent		7b
TS16: embryo ectoderm	Present		7b

Structure	Level	Pattern	Image	Note
TS18: lens vesicle epithelium	Present		7d	There is no expression in the lens fibers.
TS18: retina	Absent		7d

Structure	Level	Pattern	Image	Note
TS22: lens epithelium	Present		7f
TS22: lens fiber	Absent		7f
TS22: retina	Absent		7f

Structure	Level	Pattern	Image	Note
TS16: otocyst	Present	Uniform	8b

Structure	Level	Pattern	Image	Note
TS22: inner ear	Present	Regionally restricted	8d	Expression is present in a distinct pattern.

Structure	Level	Pattern	Image	Note
TS27: epidermis basal layer	Present		8f
TS27: epidermis granular layer	Present		8f
TS27: epidermis cornified layer	Absent		8f

Structure	Level	Pattern	Image	Note
TS22: intestine	Strong		9b
TS22: pancreas	Strong		9b
TS22: mesothelium	Absent		9b

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Present	Widespread	9d	Expression is present in all metanephric tubules.

Structure	Level	Pattern	Image	Note
TS22: visceral organ system	Strong			Expression was always strong in endodermal organs. It was not detected in any of the lateral plate derivatives.
TS22: cartilage	Absent
TS22: dermis	Absent
TS22: musculature	Absent
TS22: somite	Absent