Probe:	Gbx2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Probe length was reduced by alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	6A	Expression is in the three primitive germ layers in the posterior part of the embryo, absent in head folds.
TS11: embryo endoderm	Present	Regionally restricted	6A	Expression is in the three primitive germ layers in the posterior part of the embryo, absent in head folds.
TS11: embryo mesoderm	Present	Regionally restricted	6A	Expression is in the three primitive germ layers in the posterior part of the embryo, absent in head folds.
TS11: extraembryonic component	Absent		6A

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	6B	Expression is from the primitive srteak to the curve demarcating the early head folds.
TS11: embryo endoderm	Present	Regionally restricted	6B	Expression is in newly formed enoderm and mesoderm adjacent to the primitive streak.
TS11: embryo mesoderm	Present	Regionally restricted	6B	Expression is in newly formed enoderm and mesoderm adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present		6C
TS13: future spinal cord neural fold	Present		6C
TS13: trunk unsegmented mesenchyme	Present		6C
TS13: embryo	Present	Regionally restricted	6C	Expression is caudal.
TS13: midbrain-hindbrain junction	Strong		6C
TS13: future hindbrain	Weak		6C

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch ectoderm	Present		6D
TS13: 1st branchial arch endoderm	Present		6D
TS13: 1st branchial arch mesenchyme	Present		6D

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Strong		6E
TS15: rhombomere 4	Strong		6E
TS15: tail	Strong		6E
TS15: 1st branchial arch mandibular component	Present		6E
TS15: 1st branchial pouch	Present		6E
TS15: foregut	Present		6E
TS15: future forebrain	Present		6E
TS15: future midbrain	Present		6E
TS15: future spinal cord	Present		6E
TS15: heart	Present		6E
TS15: neural tube	Present		6E
TS15: rhombomere 2	Present		6E
TS15: rhombomere 3	Present		6E
TS15: rhombomere 7	Present		6E
TS15: otocyst	Strong	Regionally restricted	6E	Expression is at the dorsomedial epithelium.
TS15: branchial arch	Present	Regionally restricted	6E	Expression is at the interface between each arch and along the rostral aspect of the mandibular swellings.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Strong		6F
TS15: rhombomere 4	Strong		6F
TS15: future forebrain	Present		6F
TS15: future midbrain	Present		6F
TS15: future spinal cord	Present		6F
TS15: neural tube	Present		6F
TS15: rhombomere 2	Present		6F
TS15: rhombomere 3	Present		6F
TS15: rhombomere 7	Present		6F
TS15: embryo ectoderm	Present	Regionally restricted	6F	Expression is caudal.
TS15: embryo	Present	Regionally restricted	6F	Expression is posterior.
TS15: otocyst	Strong	Regionally restricted	6F	Expression is at the dorsomedial epithelium.
TS15: rhombomere 5	Absent		6F
TS15: rhombomere 6	Absent		6F