Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:35560
Zhou L, et al., Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung. J Histochem Cytochem. 1996 Oct;44(10):1183-93
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5463647
|
Gene symbol:
|
Sftpb
|
Gene name:
|
surfactant associated protein B
|
Antibody:
|
R4633
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
Assay notes:
|
Antibody recognizes the pro-SP-B. It is possible that another antibody (MGI:5462421) was used for the assay too. The author did not mention which antibody, or both antibodies were used.
|
|
Results
|
Specimen
2C:
embryonic day 10.0
(more )
Specimen
2C:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: lung |
Absent |
|
2C
|
|
Specimen
2G:
embryonic day 11.0
(more )
Specimen
2G:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: lung |
Weak |
Regionally restricted |
|
Expressed in newly formed distal tubules, but not in proximal airways.
|
Specimen
3C:
embryonic day 13.0
(more )
Specimen
3C:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 13.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: lobar bronchus epithelium |
Weak |
Regionally restricted |
3C
|
Expressed in newly formed distal tubules, but not in proximal columnar epithelial cells.
|
Specimen
3G:
embryonic day 16.0
(more )
Specimen
3G:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown:
embryonic day 17.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung alveolus |
Present |
|
|
Expressed in the differentiated Type II cells.
|
|