Probe:	D2 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Present		2A
TS9: embryo mesoderm	Present	Regionally restricted	2A	Expression was detected in the mesoderm migrating away from the primitive streak.

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Present		2C
TS9: embryo mesoderm	Present	Regionally restricted	2C	Expression was detected in the mesoderm migrating away from the primitive streak.
TS9: embryo ectoderm	Present	Regionally restricted	2C	Expression was detected in the ectoderm located in the vicinity of the primitive streak.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Absent		2D

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2E	The expression domain extended further towards the distal tip of the embryo as the streak elongates.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present		2F

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2G
TS10: embryo ectoderm	Present	Regionally restricted	2G	Expression was detected in the ectoderm in the vicinity of the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Absent		2H

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2I	The expression domain extended further towards the distal tip of the embryo as the streak elongates.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present		2J

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2K
TS10: embryo ectoderm	Present	Regionally restricted	2K	Expression was detected in the ectoderm in the vicinity of the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2L

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2M	The expression domain extended further towards the distal tip of the embryo as the streak elongates.

Structure	Level	Pattern	Image	Note
TS11: yolk sac mesoderm	Present		2N

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2O
TS11: embryo ectoderm	Present	Regionally restricted	2O	Expression was detected in ectoderm in the vicinity of the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		2P

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Strong	Regionally restricted	2Q	Expression was stronger in the posterior ectoderm and posterior mesoderm.
TS11: embryo mesoderm	Strong	Regionally restricted	2Q	Expression was stronger in the posterior ectoderm and posterior mesoderm.
TS11: yolk sac mesoderm	Present	Regionally restricted	2Q	Expression was detected in posterior visceral yolk sac mesoderm.
TS11: amnion ectoderm	Present		2Q
TS11: amnion mesoderm	Present		2Q

Structure	Level	Pattern	Image	Note
TS12: neural tube	Very strong	Regionally restricted	5A	Expression was very strong in the mid-neural tube.
TS12: rhombomere	Present	Regionally restricted	5A	Expression was detected in prospective rhomobomere 3.
TS12: primitive streak	Present	Regionally restricted	5A	Expression was detected in the caudal-most primitive streak region.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		5B	Expression increased in the 7-9 somite embryo compared to the 3-5 somite embryo.
TS12: future midbrain	Present		5B	Expression increased in the 7-9 somite embryo compared to the 3-5 somite embryo.
TS12: future prosencephalon	Present		5B	Expression increased in the 7-9 somite embryo compared to the 3-5 somite embryo.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		5C	Expression increased in the 10-12 somite embryo compared to the 3-5 somite embryo.
TS13: future midbrain	Present		5C	Expression increased in the 10-12 somite embryo compared to the 3-5 somite embryo.
TS13: future prosencephalon	Present		5C	Expression increased in the 10-12 somite embryo compared to the 3-5 somite embryo.
TS13: neural tube	Present	Regionally restricted	5C	The posterior limit of expression in the neural tube moved progressively caudally as the primitive streak regressed.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	5D	Expression was detected in the ventral midline of the midbrain.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	5E	Expression was detected in the ventral midline.
TS12: future prosencephalon	Present	Regionally restricted	5E	Expression was detected in the ventral midline.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	5F	Expression was detected in the ventral midline of the midbrain.
TS12: neural fold	Present	Regionally restricted	5F	Expression was in the dorsal tips of the neural folds at the level of rhombomere 3 in the hindbrain.
TS12: embryo mesenchyme	Present		5F
TS12: foregut diverticulum endoderm	Present		5F
TS12: amnion	Absent		5F
TS12: surface ectoderm	Present	Regionally restricted	5F	Expression was detected at the junction between the amnion and the embryo proper.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	5G	Expression was detected in the whole neuroectoderm, except for the floor plate, in the region of the closing neural tube.
TS12: neural plate	Absent		5G
TS12: surface ectoderm	Present		5G
TS12: embryo mesenchyme	Present	Regionally restricted	5G	Expression was detected at the junction between the amnion and the embryo proper.
TS12: yolk sac	Absent		5G

Structure	Level	Pattern	Image	Note
TS12: somite	Absent		5H

Structure	Level	Pattern	Image	Note
TS12: cardiac muscle tissue	Present
TS12: endocardial tube	Present
TS12: foregut diverticulum endoderm	Present
TS12: head mesenchyme	Present
TS12: hindgut diverticulum endoderm	Present
TS12: paraxial mesenchyme	Present
TS12: surface ectoderm	Present
TS12: allantois	Present	Regionally restricted		Expression was detected in the base of the allantois.
TS12: neural tube	Strong	Regionally restricted
TS12: somatopleure	Strong	Regionally restricted
TS12: neural ectoderm	Strong	Regionally restricted		Expression was strong in the neural ectoderm in the anterior region of the embryo. Expression level varied in the posterior region of the embryo.
TS12: amnion ectoderm	Absent
TS12: amnion mesenchyme	Absent
TS12: blood island	Absent
TS12: splanchnopleure	Absent
TS12: yolk sac endoderm	Absent
TS12: yolk sac mesenchyme	Absent
TS12: somite	Ambiguous

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		7F

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Strong		7G
TS13: rhombomere	Strong	Regionally restricted	7G	Expression was high in rhombomeres 6 and 7.
TS13: rhombomere 4	Weak		7G
TS13: rhombomere 5	Weak		7G
TS13: rhombomere 2	Trace		7G

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Strong		7H
TS13: rhombomere	Strong	Regionally restricted	7H	Expression was high in rhombomeres 6 and 7.
TS13: rhombomere 4	Weak		7H
TS13: rhombomere 5	Weak		7H
TS13: rhombomere 2	Trace		7H

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Strong		7I
TS13: rhombomere	Strong	Regionally restricted	7I	Expression was high in rhombomeres 6 and 7.
TS13: rhombomere 4	Weak		7I
TS13: rhombomere 5	Weak		7I
TS13: rhombomere 2	Trace		7I

Structure	Level	Pattern	Image	Note
TS15: rhombomere 3	Present		7J
TS15: rhombomere 5	Present		7J