Gene Expression Data
Assay Details
Assay
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Reference:
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J:18124
Edmondson DG, et al., Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis. Development. 1994 May;120(5):1251-63
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5461838
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Gene symbol:
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Mef2c
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Gene name:
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myocyte enhancer factor 2C
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Probe:
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Mef2c probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Authors state that two probes (MGI:5461731 and MGI:5461732) were used and gave identical in situ hybridization patterns.
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Results
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Specimen
3A:
embryonic day 8.0
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3B:
embryonic day 9.5
(more )
Specimen
3B:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 9.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: heart |
Present |
|
3B
|
|
TS15: somite |
Strong |
Regionally restricted |
3B
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High levels of expression in the rostral somites. Transcripts distributed homogenously throughout the more caudal, less mature somites. No expression in the 11 most caudal somites.
|
TS15: myotome |
Present |
Regionally restricted |
3B
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In older somites, transcripts enriched in regions between or at edges of myotomes.
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Specimen
6B:
embryonic day 10.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: heart |
Present |
|
6B
|
|
TS17: blood vessel |
Present |
Regionally restricted |
6B
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Expression cells that appear to represent blood vessels within the midbrain.
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Specimen
6C:
embryonic day 10.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: somite |
Present |
Regionally restricted |
6C
|
In older somites, transcripts enriched in regions between or at edges of myotomes.
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Specimen
6E:
embryonic day 10.5
(more )
Specimen
6E:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: heart |
Present |
|
6E
|
|
TS17: myotome |
Present |
Regionally restricted |
6E
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Expression initially localized to a group of myocytes in the medial region of the myotome. In older somites, transcripts enriched in regions between or at edges of myotomes.
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TS17: somite |
Present |
Uniform |
6E
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Transcripts distributed homogenously throughout the more caudal, less mature somites.
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Specimen
not shown day 10.5 section from whole mount:
embryonic day 10.5
(more )
Specimen
not shown day 10.5 section from whole mount:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
6G:
embryonic day 11.5
(more )
Specimen
6G:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: somite |
Present |
Regionally restricted |
6G
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Expression beginning to decline in rostral somites.
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Specimen
not shown day 11.5:
embryonic day 11.5
(more )
Specimen
not shown day 11.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown day 11.5 section from whole mount:
embryonic day 11.5
(more )
Specimen
not shown day 11.5 section from whole mount:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: arterial system |
Present |
Regionally restricted |
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Expression in columns of cells that appear to be developing intercostal arteries.
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