Probe:	Mef2c probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Authors state that two probes (MGI:5461731 and MGI:5461732) were used and gave identical in situ hybridization patterns.

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: heart	Present	Regionally restricted	1B	Expression in a localized region of thickened mesothelial cells in the anterior region of the embryo, representing cardiac mesoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present		1D

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber	Present		1F
TS13: primitive ventricle	Present		1F
TS13: neural crest	Present		1F

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Strong		2B	High levels of expression in myocardiogenic progenitor cells.

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Strong		2D	High levels of expression in myocardiogenic progenitor cells.

Structure	Level	Pattern	Image	Note
TS14: myotome	Present	Regionally restricted		Expression in the myotomes of the rostral-most somites.

Structure	Level	Pattern	Image	Note
TS15: heart	Present		4B
TS15: somite	Present	Regionally restricted	4B	Expression in the myotomes of the rostral-most somites.

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		5B
TS17: heart	Present		5B
TS17: blood vessel	Present	Regionally restricted	5B	Expression in what appear to be blood vessels forming around the neural tube.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present

Structure	Level	Pattern	Image	Note
TS19: heart	Present

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		7A
TS19: hindlimb bud	Present		7A
TS19: limb	Present	Regionally restricted	7A	Expression in differentiating skeletal muscle fibers throughout the limbs and trunk.
TS19: trunk	Present	Regionally restricted	7A	Expression in differentiating skeletal muscle fibers throughout the limbs and trunk.
TS19: gut	Weak	Regionally restricted	7A	Low levels of expression in cells contributing to smooth muscle of gut.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Strong	Regionally restricted	8B	High levels of expression in skeletal muscles throughout the embryo. Highest levels of expression near terminal regions of muscle fibers.
TS21: tail mesenchyme derived from neural crest	Present	Regionally restricted	8B	Expression in neural crest derivatives in the frontonasal region.

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Weak
TS21: vertebral cartilage condensation	Weak

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle	Weak			Authors state that the level of expression in skeletal muscle has declined.