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Gene Expression Data
Assay Details
Assay
Reference: J:177278 Chang SL, et al., Region- and cell type-selective expression of the evolutionarily conserved Nolz-1/zfp503 gene in the developing mouse hindbrain. Gene Expr Patterns. 2011 Dec;11(8):525-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5448251
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-C-Isl-1 protein (K5)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: The antibody used in this assay recognizes two proteins.
Results
Specimen 4A1: embryonic day 18.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Zfp503 (assay MGI:5448161).
Structure Level Pattern Image Note
TS26: hindbrain Present 4A1
Specimen 4A2: embryonic day 18.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Zfp503 (assay MGI:5448161).
Structure Level Pattern Image Note
TS26: pons Present Regionally restricted 4A2 Expression is present in the trigeminal nerve.
Specimen 4A3: embryonic day 18.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Zfp503 (assay MGI:5448161).
Structure Level Pattern Image Note
TS26: pons Present 4A3
Specimen 4B3: embryonic day 18.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Zfp503 (assay MGI:5448161).
Structure Level Pattern Image Note
TS26: pons Present 4B3
Specimen 4D: embryonic day 18.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Zfp503 (assay MGI:5448161).
Structure Level Pattern Image Note
TS26: medulla oblongata Present 4D1 , 4D2 Expression is present in somatic motor neurons, such as in the nucleus of the hypoglossal cranial nerve. Once such cell is pictured in the inset.
Specimen 5A: embryonic day 10.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Shh (assay MGI:5448260).
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 5A Expression is present in the motor neurons.
Specimen 5B: embryonic day 10.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Phox2b (assay MGI:5448261).
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 5B Expression is present in motor neurons. A caudally migrating branchial/visceral motor neuron is pictured in the inset.
Specimen 5b1: embryonic day 10.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Phox2b (assay MGI:5448261).
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 5Bb1 Expression is present in motor neurons.
Specimen 5b2: embryonic day 10.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Zfp503 (assay MGI:5448161).
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 5Bb2 Expression is present in motor neurons.
Specimen 5D: embryonic day 11.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Shh (assay MGI:5448260).
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 5D Expression is present in motor neurons, including somatic motor neurons.
Specimen 5E: embryonic day 11.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Zfp503 (assay MGI:5448161).
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 5E Expression is present in somatic motor neurons.
Specimen 5F: embryonic day 11.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Phox2b (assay MGI:5448261).
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 5F Expression is present in branchial motor neurons.
Specimen 5G/H: embryonic day 11.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Shh (assay MGI:5448260).
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 5H , 5G Expression is present in somatic motor neurons. One of these expressing neurons from the medial part of the hindbrain at the level caudal to the optic vesicle is pictured in the inset.
Specimen 5I: embryonic day 11.5 (more )
Note: Double labeled: brown - Isl1 and Isl2; blue - Phox2b (assay MGI:5448261).
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 5I Expression is present in motor neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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