Probe:	Auts2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS18: brain	Absent		2A	Expression barely or not detected at all.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2B

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Strong	Regionally restricted	2C	Expressed as a homogeneous thin layer along the rostro-caudal axis.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	2D	Expressed as a homogeneous thin layer along the rostro-caudal axis.
TS22: thalamus	Strong	Regionally restricted	2D	Expressed in developing dorsal thalamus.
TS22: brainstem	Strong	Regionally restricted	2D	Expressed in the locus ceruleus.
TS22: cerebellum	Strong		2D	Expressed in the precursors of Purkinje cells, deep nuclei, and granule neurons.
TS22: cerebellum mantle layer	Strong		2D
TS22: hippocampus	Present	Regionally restricted	2D	Expressed in the hippocampal primordium, but not in the cortical hem or fimbria.

Structure	Level	Pattern	Image	Note
TS22: pons	Strong	Regionally restricted		Expressed in the vestibular nuclei.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong		2d
TS22: hippocampus	Present		2d	Expressed in the hippocampal primordium, but not in the cortical hem or fimbria.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Graded	2E	A graded expression, with frontal cortical regions exhibiting high expression in deep and superficial layers. Only weaker expression in the deeper cortical layers in caudal cortical regions.
TS24: hippocampus	Present		2E

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong		2e

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong	Regionally restricted	2F	Only expressed in the rostral region of the cortex, more localized to the Purkinje cell layer.
TS26: cerebellum Purkinje cell layer	Present		2F
TS26: pretectum	Present		2F
TS26: inferior colliculus	Present		2F
TS26: superior colliculus	Present		2F
TS26: dentate gyrus	Present	Regionally restricted	2F	Expressed in both the suprapyramidal and infrapyramidal blades, throughout the hilus.
TS26: hippocampus region	Present		2F	Expressed in the CA fields.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	2G	Only expressed in the rostral region of the cortex, restricted to superficial layers.
TS28: anterior thalamic group	Strong	Regionally restricted	2G	Expressed in the anterior thalamic nuclei.
TS28: basal ventral medial thalamic nucleus	Strong		2G
TS28: lateral thalamic group	Strong	Regionally restricted	2G	Expressed in the ventrolateral nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak	Regionally restricted	2h" , 2H	Only expressed in the rostral region of the cortex, and very superficial layers in frontal cortex.
TS28: hippocampus region	Present		2H	Expressed in the CA fields.
TS28: dentate gyrus granule cell layer	Present		2H
TS28: cerebellum Purkinje cell layer	Present		2H , 2h'
TS28: dorsal thalamus	Present		2H	Expression is markedly decreased.
TS28: inferior colliculus	Present	Scattered	2H
TS28: pretectum	Present	Scattered	2H
TS28: superior colliculus	Present	Scattered	2H

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		3A , 3a	Expressed in postmitotic neurons (marked by Tbr1).

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		3B , 3b	Not expressed in the Cajal-Retzius cells (marked by Calretinin).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		3C , 3c	Expressed in a subset of the radial glial progenitors (marked by Pax6).
TS22: cerebral cortex ventricular layer	Present		3C , 3c	Expressed in a subset of the radial glial progenitors (marked by Pax6).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		3d , 3D	Expressed in a subset of the intermediate neuronal progenitors (marked by Tbr2).
TS22: cerebral cortex ventricular layer	Present		3d , 3D	Expressed in a subset of the intermediate neuronal progenitors (marked by Tbr2).

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Strong		3E , 3e	Expressed in majority of the neurons (marked by Tbr1).
TS22: cerebral cortex intermediate zone	Ambiguous		3E , 3e	Weak or no staining at all.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		3f , 3F	Colocalized with Ctip2, a marker for cortical plate.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3G	Expressed superficially in the frontal cortex.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3H	Expressed superficially in the frontal cortex.
TS27: cortical layer V	Present		3H	Coexpression with Ctip2.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3I	Expressed superficially in the frontal cortex. Coexpression with Cux1, an upper layer marker.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3L	Restricted to a small subset of cortical neurons (marked by Tbr1).

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present		4A
TS27: hippocampus region	Present		4A

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present		4B	Colocalization with Tbr1 in the majority of cells.
TS27: hippocampus region	Present		4B	Colocalization with Tbr1 in the majority of cells.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	5a , 5A	High expression in the cortical transitory zone and the ventricular zone. Weak expression in rhombic lip, the external granular layer, and the nuclear transitory zone.
TS22: cerebellum ventricular layer	Strong		5A

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5B	No coexpression with Tbr1.
TS28: deep cerebellar nucleus	Present	Scattered	5B	Presumably GABAergic interneurons, with no coexpression for Tbr1.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Regionally restricted	5C	Expressed in developing dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		5D	Expressed in the periglomerular cells. Coexpression with Tbr1 was seen in a subset of cells.
TS28: main olfactory bulb mitral cell layer	Present		5D	Coexpression with Tbr1 was seen in a subset of cells.
TS28: main olfactory bulb granule cell layer	Trace	Scattered	5D	Rare expression was seen, possibly in glutamatergic precursors.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		5d	Expressed in the periglomerular cells.
TS28: main olfactory bulb mitral cell layer	Present		5d
TS28: main olfactory bulb granule cell layer	Present		5d	Coexpressed with Gad67 in a subset of cells.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Strong		5E	Coexpressed with TH. Higher magnification was shown in the inset.
TS28: ventral tegmental area	Strong		5E	Coexpressed with TH. Higher magnification was shown in the inset.