Probe:	cbfa1 cDNA9
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: humerus pre-cartilage condensation	Present	Regionally restricted	1A cbfa1	Expression limited to small group of cells of proximal presumptive humerus.

Structure	Level	Pattern	Image	Note
TS21: humerus cartilage condensation	Present		1B cbfa1	Expresssion in chondrocytes of humerus, radius and ulna.
TS21: radius-ulna cartilage condensation	Present		1B cbfa1	Expresssion in chondrocytes of humerus, radius and ulna.
TS21: forelimb digit pre-cartilage condensation	Absent		1B cbfa1	No expression detected in pre-chondrogenic cells of the digits.
TS21: forelimb mesenchyme	Present	Regionally restricted	1B cbfa1	No expression detected in pre-chondrogenic cells of the digits.

Structure	Level	Pattern	Image	Note
TS22: forelimb mesenchyme	Strong	Regionally restricted	1C cbfa1	Strong expression in perichondrium.
TS22: forelimb cartilage condensation	Present	Regionally restricted	1C cbfa1	Lower expression in hypertrophic chondrocytes.
TS22: radius cartilage condensation	Present	Regionally restricted	1C cbfa1	Expression in central region of cartilaginous radius, ulna and digits.
TS22: ulna cartilage condensation	Present	Regionally restricted	1C cbfa1	Expression in central region of cartilaginous radius, ulna and digits.

Structure	Level	Pattern	Image	Note
TS26: forelimb mesenchyme	Strong	Regionally restricted	2A cbfa1	Strong expression in perichondrium and periosteum, in mature, early hypertrophic and hypertrophic chondrocytes, and in newly formed bone of primary ossification center.
TS26: forelimb skeleton	Strong	Regionally restricted	2A cbfa1	Strong expression in perichondrium and periosteum, in mature, early hypertrophic and hypertrophic chondrocytes, and in newly formed bone of primary ossification center.

Structure	Level	Pattern	Image	Note
TS26: forelimb mesenchyme	Strong	Regionally restricted	2B mmp13/cbfa1	Abundant expression in perichondrium/periosteum, in hypertrophic chondrocytes and in newly formed bone.
TS26: forelimb skeleton	Strong	Regionally restricted	2B mmp13/cbfa1	Abundant expression in perichondrium/periosteum, in hypertrophic chondrocytes and in newly formed bone.

Structure	Level	Pattern	Image	Note
TS26: forelimb mesenchyme	Strong	Regionally restricted	2B cbfa1/oc	Expression in periosteum and in primary ossification center.
TS26: forelimb skeleton	Strong	Regionally restricted	2B cbfa1/oc	Expression in periosteum and in primary ossification center.

Structure	Level	Pattern	Image	Note
TS26: forelimb mesenchyme	Strong	Regionally restricted	2C cbfa1	Expression in chondrocytes, bone and periosteum.
TS26: forelimb skeleton	Strong	Regionally restricted	2C cbfa1	Expression in chondrocytes, bone and periosteum.

Structure	Level	Pattern	Image	Note
TS28: epiphyseal plate hypertrophic zone	Weak	Regionally restricted	2D cbfa1	Low levels of expression in mature and hypertrophic chondrocytes in both growth plate and secondary ossification center. Expression in hypertrophic chondrocytes in secondary ossification center, coincident with Col10a1.
TS28: tibia	Weak	Regionally restricted	2D cbfa1	Low levels of expression in mature and hypertrophic chondrocytes in both growth plate and secondary ossification center. Expression in hypertrophic chondrocytes in secondary ossification center, coincident with Col10a1.
TS28: trabecular bone	Strong	Regionally restricted	2D cbfa1	Expression in bony trabeculae of primary growth plate. Stronger expression in regions of new bone formation.