Probe:	clone 15B
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: extraembryonic mesenchyme	Present		1A/B
TS12: lateral plate mesoderm	Present		1A/B

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present		1E/F
TS13: somatopleure	Present	Regionally restricted	1E/F	Expression in somatopleure near dorsal aorta.
TS13: amnion	Present	Regionally restricted	1E/F	Expression in mesodermal layer of amnion and visceral yolk sac mesoderm.
TS13: yolk sac mesenchyme	Present	Regionally restricted	1E/F	Expression in mesodermal layer of amnion and visceral yolk sac mesoderm.
TS13: head mesenchyme	Absent		1E/F	No expression in head or cardiac mesenchyme.
TS13: heart	Absent		1E/F	No expression in head or cardiac mesenchyme.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present	Regionally restricted	1I/J	Expression in splanchnic mesoderm adjacent to foregut endoderm.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	2A/B	Expression in splanchnic mesoderm adjacent to foregut and midgut endoderm.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Strong	Regionally restricted	2E/F	Abundant expression in splanchnic mesoderm juxtaposed to the lung bud.
TS15: mesenchyme derived from somatopleure	Present	Regionally restricted	2E/F	Expression in mesenchyme abutting the dorsal aorta, but not completely surrounding the artery.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	2G/H	Expression in splanchnic mesoderm lining the entire midgut endoderm.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	3A/B	Expression in splanchnic mesoderm juxtaposed to foregut.

Structure	Level	Pattern	Image	Note
TS15: yolk sac mesenchyme	Present	Regionally restricted	3E/F	Uniform expression in yolk sac mesoderm of the embryo proper side of the blood island vessel.

Structure	Level	Pattern	Image	Note
TS21: embryo mesenchyme	Present	Regionally restricted	4A/B	Expression restricted to mesenchyme derived from lateral mesoderm.
TS21: oral region	Present	Regionally restricted	4A/B	Expression in mesenchyme of oral cavity and epiglottis
TS21: esophagus mesenchyme	Present		4A/B
TS21: incisor mesenchyme	Present		4A/B
TS21: intestine mesenchyme	Present		4A/B
TS21: lung mesenchyme	Present		4A/B
TS21: stomach mesenchyme	Present		4A/B
TS21: tongue mesenchyme	Present		4A/B
TS21: trachea mesenchyme	Present		4A/B
TS21: pharynx	Present	Regionally restricted	4A/B	Expression in mesenchyme of oropharynx,
TS21: dorsal aorta	Absent		4A/B	Expression in mesenchymal cells of dorsal aorta and intersomitic arteries.
TS21: intersegmental artery	Absent		4A/B	Expression in mesenchymal cells of dorsal aorta and intersomitic arteries.
TS21: brain vascular element	Absent		4A/B
TS21: heart	Absent		4A/B
TS21: liver	Absent		4A/B
TS21: metanephros	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present	Regionally restricted	4C/D	Higher levels of expression in mesenchymal cells adjacent to respiratory bronchioles.
TS21: lung epithelium	Absent		4C/D	No expression in endoderm-derived bronchiolar epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: stomach mesenchyme	Present		4E/F
TS21: stomach epithelium	Absent		4E/F

Structure	Level	Pattern	Image	Note
TS21: intestine mesenchyme	Present	Regionally restricted		Expression in mesenchymal cells of small intestine and colon.

Structure	Level	Pattern	Image	Note
TS24: intestine mesenchyme	Present		5A/B
TS24: lung mesenchyme	Present		5A/B
TS24: stomach mesenchyme	Present		5A/B

Structure	Level	Pattern	Image	Note
TS24: oral region	Present	Regionally restricted		Expression in mesenchyme of mouth.
TS24: incisor mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present	Regionally restricted	5C/D	Expression in mesenchymal cells of lung adjacent to respiratory bronchioles.

Structure	Level	Pattern	Image	Note
TS24: intestine mesenchyme	Strong		5G/H	Expression in mesenchymal cells of lung adjacent to respiratory bronchioles.
TS24: intestine muscularis	Strong		5G/H	Expression in mesenchymal cells of lung adjacent to respiratory bronchioles.
TS24: stomach mesenchyme	Strong		5G/H	Expression in mesenchymal cells of lung adjacent to respiratory bronchioles.

Structure	Level	Pattern	Image	Note
TS28: alveolar sac	Present		7A/B
TS28: lung vascular element	Present	Regionally restricted	7A/B	Expression in endothelial cells lining pulmonary arterioles.

Structure	Level	Pattern	Image	Note
TS28: intestine wall	Present	Regionally restricted	7G/H	Expression restricted to fibroblasts and smooth muscle cells of lamina propria of villi.
TS28: intestine smooth muscle	Present	Regionally restricted	7G/H	Expression restricted to fibroblasts and smooth muscle cells of lamina propria of villi.
TS28: intestine epithelium	Absent		7G/H	No expression in epithelium lining intestinal villi or in crypts of Lieberkuhn.