Gene Expression Data
Assay Details
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Assay
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Reference:
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J:71419
Reddy S, et al., Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis. Mech Dev. 2001 Sep;107(1-2):69-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5445570
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Gene symbol:
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Wnt5a
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Gene name:
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wingless-type MMTV integration site family, member 5A
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Probe:
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Wnt5a cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
3C:
embryonic day 14.5
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: dermis |
Present |
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3C
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| TS22: integumental system |
Present |
Regionally restricted |
3C
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Expression was detected in the dermis. Specific expression was not observed in hair follicles at the placode stage.
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Specimen
4C:
embryonic day 14.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4F:
embryonic day 18.5
(more )
Specimen
4F:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
4F
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At the hair germ stage, expression was elevated in the dermal condensate. More advanced follicles gave the strongest signals.
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Specimen
4I:
embryonic day 18.5
(more )
Specimen
4I:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
4I
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At the peg stage, expression was elevated in the dermal condensate. More advanced follicles gave the strongest signals.
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Specimen
4L:
embryonic day 18.5
(more )
Specimen
4L:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
4L
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At the bulbous peg stage, expression was strong in the dermal papilla and at lower levels in follicular epithelial cells.
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| TS26: dermis |
Weak |
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4L
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Specimen
5F:
postnatal day 7
(more )
Specimen
5F:
(close )
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Genetic Background:
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BALB/c
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7C:
embryonic day 18.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
7C
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Expression was detected in the dermal condensate of germ stage follicles.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Absent |
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7D
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Expression was not detected in the dermal condensate of germ stage follicles in the mutant.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hair follicle |
Absent |
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Expression was not detected in placode stage hair follicles.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hair follicle |
Absent |
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Expression was not detected in placode stage hair follicles as in wild type.
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Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hair follicle |
Present |
Regionally restricted |
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Expression was detected in the dermal condensate of hair follicles.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hair follicle |
Absent |
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Expression was not detected in the dermal condensate of hair follicles in the mutant.
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Analysis Tools