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Gene Expression Data
Assay Details
Assay
Reference: J:57828 Tanaka M, et al., Parental origin-specific expression of Mash2 is established at the time of implantation with its imprinting mechanism highly resistant to genome-wide demethylation. Mech Dev. 1999 Sep;87(1-2):129-42
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5444278
Gene symbol: Ascl2
Gene name: achaete-scute family bHLH transcription factor 2
Reporter: lacZ
Direct Detection
Assay notes: This is an imprinted gene, maternal or paternal expression is indicated for each specimen.
Results
Specimen 1E: embryonic day 9.5; Ascl2tm1Alj/Ascl2tm1Alj (more )
Structure Level Pattern Image Note
TS28: placenta Present 1E
TS15: embryo Present 1E Ectopic expression in this mutant.
Specimen 3A: embryonic day 5.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS8: ectoplacental cone Strong Regionally restricted 3A Maternal expression in a proximal part .
Specimen 3B: embryonic day 6.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS9: ectoplacental cone Present 3B Maternal expression.
TS9: extraembryonic ectoderm Present 3B Maternal expression.
Specimen 3C: embryonic day 7.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS11: chorion Present 3C Maternal expression.
TS11: ectoplacental cone Present 3C Maternal expression.
Specimen 3D: embryonic day 8.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS13: chorion Present 3D Maternal expression.
TS13: ectoplacental cone Present 3D Maternal expression.
Specimen 3E: embryonic day 9.0; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS14: chorioallantoic placenta Present 3E
TS14: spongiotrophoblast Present 3E
Specimen 3F: embryonic day 9.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS15: spongiotrophoblast Present 3F
TS15: embryo Absent 3F
Specimen 3G: embryonic day 5.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS8: ectoplacental cone Weak Uniform 3G Paternal expression.
Specimen 3H: embryonic day 6.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS9: ectoplacental cone Weak Regionally restricted 3H Paternal expression is restricted to a small number of cells.
Specimen 3I: embryonic day 7.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS11: ectoplacental cone Weak Regionally restricted 3I Paternal expression is restricted to a small number of cells.
Specimen 3J: embryonic day 8.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS13: ectoplacental cone Weak Regionally restricted 3J Paternal expression is restricted to a small number of cells.
Specimen 3K: embryonic day 9.0; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS14: chorioallantoic placenta Weak 3K Paternal expression.
Specimen 3L: embryonic day 9.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS15: spongiotrophoblast Weak 3L Paternal expression.
Specimen 4A: embryonic day 6.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele, section of specimen 3B.
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 4A Maternal expression.
TS9: trophectoderm Present 4A Maternal expression.
Specimen 4B: embryonic day 7.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele, section of specimen 3C.
Structure Level Pattern Image Note
TS11: chorion Present 4B Maternal expression.
TS11: ectoplacental cone Present 4B Maternal expression.
Specimen 4C: embryonic day 8.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele, section of specimen 3D.
Structure Level Pattern Image Note
TS13: chorion Present 4C Maternal expression.
TS13: ectoplacental cone Present 4C Maternal expression.
Specimen 4D: embryonic day 6.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele, section of specimen 3H.
Structure Level Pattern Image Note
TS9: ectoplacental cone Present Regionally restricted 4D Paternal expression.
Specimen 4E: embryonic day 7.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele, section of specimen 3I.
Structure Level Pattern Image Note
TS11: ectoplacental cone Present 4E Paternal expression.
Specimen 4F: embryonic day 8.5; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Paternal allele, section of specimen 3J.
Structure Level Pattern Image Note
TS13: ectoplacental cone Present Regionally restricted 4F Paternal expression. Expression is restricted to the external region.
Specimen 5C: embryonic day 10.0; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS16: spongiotrophoblast Present 5C Maternal allele. The number of cells is reduced.
TS28: placenta Present 5C Maternal allele. The number of cells is reduced.
Specimen 5D: embryonic day 10.0; Ascl2tm1.1Nagy/Ascl2+ (more )
Note: Maternal allele. Double labeled: blue - Ascl2 reporter; brown - Tpbpa (assay MGI:5444296).
Structure Level Pattern Image Note
TS16: spongiotrophoblast Present 5D Maternal allele, The number of cells is reduced.
TS28: placenta Present 5D Maternal allele, The number of cells is reduced.
Specimen Not shown E10 paternal: embryonic day 10.0; Ascl2tm1.1Nagy/Ascl2+ (more )
Structure Level Pattern Image Note
TS16: spongiotrophoblast Present Paternal epression. This is similar to wild-type.
TS28: placenta Present Paternal epression. This is similar to wild-type.
Specimen 6G: embryonic day 8.5; Ascl2tm1Alj/Ascl2+, Dnmt1tm1Enl/Dnmt1tm1Enl (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS28: placenta Present 6G Maternal allele. Expression is similar as wild-type.
Specimen 6H: embryonic day 8.5; Ascl2tm1Alj/Ascl2+, Dnmt1tm1Enl/Dnmt1tm1Enl (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS28: placenta Present 6H Paternal allele. Levels were lower.
Specimen 6K: embryonic day 8.5; Ascl2tm1Alj/Ascl2+, Dnmt1tm1Enl/Dnmt1tm1Enl (more )
Note: Maternal allele
Structure Level Pattern Image Note
TS13: ectoplacental cone Present 6K Maternal allele.
Specimen 6L: embryonic day 8.5; Ascl2tm1Alj/Ascl2+, Dnmt1tm1Enl/Dnmt1tm1Enl (more )
Note: Paternal allele
Structure Level Pattern Image Note
TS13: ectoplacental cone Weak 6L Paternal allele.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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