Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39657
Lobe CG, Expression of the helix-loop-helix factor, Hes3, during embryo development suggests a role in early midbrain-hindbrain patterning. Mech Dev. 1997 Mar;62(2):227-37
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5444149
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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En1 cDNA6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3C:
embryonic day 8.5
(more  )
Note: 7-8 somite stage embryo.
Specimen
3C:
(close  )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7-8 somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
Regionally restricted |
3C
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Expression is in the region of the mesencephalon/metencephalon boundary, and extends ventrally to the floor plate.
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| TS14: future midbrain |
Present |
Regionally restricted |
3C
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Expression is in the region of the mesencephalon/metencephalon boundary, and extends ventrally to the floor plate.
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| TS14: midbrain-hindbrain junction |
Present |
Regionally restricted |
3C
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Expression is in the region of the mesencephalon/metencephalon boundary, and extends ventrally to the floor plate.
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Specimen
4L:
embryonic day 10.0
(more )
Specimen
4L:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future midbrain |
Strong |
Regionally restricted |
4L
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Expressed in the inferior colliculus.
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| TS16: future hindbrain |
Strong |
Regionally restricted |
4L
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Expressed in metencephalon neighboring the mesencephalic isthmus.
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Analysis Tools