Gene Expression Data
Assay Details
Assay
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Reference:
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J:29060
Bally-Cuif L, et al., Involvement of Wnt-1 in the formation of the mes/metencephalic boundary. Mech Dev. 1995 Sep;53(1):23-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5441617
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 probe23
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.0
(more )
Note: 3-5 somites.
Specimen
2A:
(close )
Genetic Background:
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either: C57BL/6J or OF1
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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Structure |
Level |
Pattern |
Image |
Note |
TS12: future brain |
Present |
Regionally restricted |
2A
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Uniform expression in the anterior neural tube. Authors describe a fuzzy posterior limit of expression.
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Specimen
2D:
embryonic day 8.0
(more )
Note: 7 somites.
Specimen
2D:
(close )
Genetic Background:
|
either: C57BL/6J or OF1
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
7 somites.
|
Specimen
2E:
embryonic day 8.5
(more )
Note: 8 somites.
Specimen
2E:
(close )
Genetic Background:
|
either: C57BL/6J or OF1
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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8 somites.
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Specimen
2F:
embryonic day 9.0
(more )
Specimen
2F:
(close )
Genetic Background:
|
either: C57BL/6J or OF1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: future hindbrain |
Present |
Regionally restricted |
2F
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No expression on the edge of rhombomere 1 and cerebellar plate
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TS14: rhombomere 1 |
Present |
Regionally restricted |
2F
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No expression on the edge of rhombomere 1 and cerebellar plate
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|