Probe:	Otx2 probe23
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		1A	Motoneurons of cranial nerve nuclei III and IV are absent. Scattered immunoreactive cells, possibly one or two spared nerve IV motoneurons.
TS17: hindbrain	Present	Regionally restricted	1A	Expression in a row of cells in the adjacent rhombencephalon at the edge of the cerebellar plate

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		1C	Expression in the anterior neural tube up to a caudal limit at the midbrain-hindbrain junction.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	2B	Uniform expression in the anterior neural tube. Authors describe a fuzzy posterior limit of expression.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		4A

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted	4B	Expression in the anterior neural tube up to a caudal limit at the midbrain-hindbrain junction.
TS17: rhombomere 1	Present	Regionally restricted	4B	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.
TS17: rhombomere 2	Absent		4B	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.
TS17: hindbrain	Present	Regionally restricted	4B	Abnormal pattern of expression at the choroid plexus/cerebellar limit.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted	4C	Expression in the mes/metencephalic region. Mixing of positive and negative expression domains.
TS17: rhombomere 1	Present	Regionally restricted	4C	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2
TS17: rhombomere 2	Absent		4C	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted	4D	Expression in the mes/metencephalic region. Mixing of positive and negative expression domains.
TS17: rhombomere 1	Present	Regionally restricted	4D	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.
TS17: rhombomere 2	Absent		4D	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 1	Present	Regionally restricted	4E	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.
TS17: rhombomere 2	Absent		4E	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	4F	Abnormal pattern of expression at the choroid plexus/cerebellar limit. Expression in ectopic patches and at the edge of the cerebellar plate are in mirror image.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		5G
TS19: midbrain	Present		5G

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		5H	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		5I	Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.