Probe:	Pax8 cDNA7
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Weak		3K	Expressed in the midbrain-hindbrain boundary. Expression at this area appeared a little stronger than in other regions.
TS15: future brain	Weak	Regionally restricted	3K	Expressed throughout the neuroepithelium of the brain.
TS15: otocyst	Absent		3K	No expression in the otic vesicles.

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Weak		3L
TS19: myelencephalon	Strong		3L
TS19: cerebellum primordium	Weak	Regionally restricted	3L	Expressed at the tip of the cerebellum.

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present		3M	Expression appeared to be restricted by the rhombic isthmus, without extending into the mesencephalon.
TS19: midbrain	Absent		3M

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present		4J
TS19: midbrain	Absent		4J	No expression in mesencephalon.

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Weak	Regionally restricted	4K	Expressed in the lateral region.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon	Strong		4L
TS19: neural retinal epithelium	Absent		4L
TS19: optic stalk	Absent		4L

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present		5L	Expressed in the myelencephalon.
TS21: metencephalon	Weak		5L

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present		5M	Expressed in the myelencephalon.
TS21: metencephalon	Weak		5M

Structure	Level	Pattern	Image	Note
TS23: midbrain-hindbrain junction	Present	Spotted	5N	Expressed in the midbrain-hindbrain boundary, in the region between the cerebellum and the inferior colliculus.
TS23: midbrain	Present	Spotted	5N	Expressed in the mesencephalic flexure.
TS23: medulla oblongata	Present		5N	Expressed in the myelencephalon.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present		6L
TS19: neural tube lateral wall	Present	Regionally restricted	6L	Expressed in the lateral margin of the ventricular zone of both alar and basal plates and also in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Strong		6M

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Absent		6N
TS19: inner ear	Absent		6N	No expression in the otic vesicle.