Probe:	Gdnf cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: tail neural tube	Present	Regionally restricted		Expression is present in the ventricular zone and the dorsal portion of the neural tube.
TS15: tail paraxial mesenchyme	Present	Regionally restricted		Expression is present in the dorsolateral portion of the somites in the prospective dermomyotme and lateral body wall.
TS15: future spinal cord	Present	Regionally restricted		Expression is present in the dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS17: hindbrain lateral wall	Strong	Regionally restricted	3A/B	Expression is present in two discrete regions in the rostral and caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS19: hindbrain lateral wall	Strong	Regionally restricted	3C/D	Expression is present in two discrete regions in the rostral and caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	Strong	Regionally restricted	3E/F	Expression is present in two discrete regions in the rostral and caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS22: hindbrain lateral wall	Strong	Regionally restricted	3G/H	Expression is present in two discrete regions in the rostral and caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS14: gut mesenchyme	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the cloaca. This includes the stomach and duodenum.

Structure	Level	Pattern	Image	Note
TS17: midgut mesenchyme	Strong		4A/B

Structure	Level	Pattern	Image	Note
TS17: gut mesenchyme	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the cloaca. This includes the stomach and duodenum.
TS17: 1st branchial arch	Strong			Part of the expresssing area develops into the tubo-tympanic recess.

Structure	Level	Pattern	Image	Note
TS19: esophagus mesenchyme	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the rectum.
TS19: gut mesenchyme	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the rectum.
TS19: hindgut mesenchyme	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the rectum.
TS19: physiological umbilical hernia	Present
TS19: metanephros	Present

Structure	Level	Pattern	Image	Note
TS22: esophagus mesenchyme	Strong		4C/D

Structure	Level	Pattern	Image	Note
TS22: stomach mesenchyme	Present		4E/F

Structure	Level	Pattern	Image	Note
TS22: intestine mesenchyme	Present		4G/H
TS22: rectum mesenchyme	Present		4G/H

Structure	Level	Pattern	Image	Note
TS23: esophagus mesenchyme	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the rectum.
TS23: gut muscularis	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the rectum.
TS23: rectum mesenchyme	Strong			There is a strong signal over the mesenchymal layer of the gastrointestinal tract, from the esophagus to the rectum.
TS23: physiological umbilical hernia	Present
TS23: nose	Present	Regionally restricted		Expression is present in the connective tissue mesenchyme surrounding the primordia of the vibrissae follicles.
TS23: hindbrain lateral wall	Present	Regionally restricted		Expression is present in two discrete regions in the rostral and caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS21: renal cortex	Present		5A/B	Expression in the kidney is restricted to the undifferentiated cortical mesenchymal tissue adjacent to the condensing mesenchyme surrounding the ureteric buds.
TS21: renal tubule	Absent		5A/B
TS21: renal vesicle	Absent		5A/B
TS21: gland	Absent		5A/B	There is no expression in the adrenal gland.

Structure	Level	Pattern	Image	Note
TS22: renal cortex	Present		5C/D	Expression in the kidney is restricted to the undifferentiated cortical mesenchymal tissue adjacent to the condensing mesenchyme surrounding the ureteric buds.
TS22: adrenal gland	Absent		5C/D
TS22: renal tubule	Absent		5C/D
TS22: renal vesicle	Absent		5C/D

Structure	Level	Pattern	Image	Note
TS23: renal cortex	Present		5E/F	Expression in the kidney is restricted to the undifferentiated cortical mesenchymal tissue adjacent to the condensing mesenchyme surrounding the ureteric buds.
TS23: adrenal gland	Absent		5E/F
TS23: renal tubule	Absent		5E/F
TS23: renal vesicle	Absent		5E/F

Structure	Level	Pattern	Image	Note
TS23: seminiferous cord	Present		5G/H	Expression in the testes is restricted to the periphery of seminiferous tubules (where the precursors of Sertoli cells are found).
TS23: germ cell of testis	Absent		5G/H
TS23: interstitium of the testis	Absent		5G/H

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	6A/B	Expression is present in the posterior region of the limb buds, in the zone of polarizing activity.

Structure	Level	Pattern	Image	Note
TS23: digit mesenchyme	Strong		6C/D	Expression is present in the pericartilage digital mesenchyme, the mesenchymal tissue surrounding the cartilaginous components of the developing digits.

Structure	Level	Pattern	Image	Note
TS23: femur	Present		6E/F	Expression is present in the pericartilage mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: intercostal muscle	Present		6G/H	Expression is present in the pericartilage mesenchyme of the costae.

Structure	Level	Pattern	Image	Note
TS22: nose	Present	Regionally restricted	7A/B	Expression is present in the connective tissue mesenchyme surrounding the primordia of the vibrissae follicles.
TS22: ethmoid bone primordium	Present		7A/B	Expression is present in the pericartilage mesenchyme of the cribiform plate.

Structure	Level	Pattern	Image	Note
TS22: tongue mesenchyme	Present		7C/D	Expression is present in the mesenchyme underlying the tongue.
TS22: lower jaw mesenchyme	Present		7C/D	Expression is present in the mesenchyme underlying the mandibular epithelium.
TS22: jaw mesenchyme	Present	Regionally restricted	7C/D	Expression is present in the mesenchyme surrounding the incisor primordium.
TS22: incisor mesenchyme	Present		7C/D	Expression is present in tooth bud mesenchyme.
TS22: foregut mesenchyme	Strong	Regionally restricted	7C/D	Expression is present in mesenchyme surrounding the entrance of the esophagus.

Structure	Level	Pattern	Image	Note
TS22: lens	Present		7E/F
TS22: eye	Present	Regionally restricted	7E/F	Expression is present in the optical cup, presumably in the mesenchyme.
TS22: optic nerve	Absent		7E/F
TS22: retina	Absent		7E/F

Structure	Level	Pattern	Image	Note
TS22: inner ear mesenchyme	Present		7G/H	Expression is present in mesenchyme around the acoustic epithelium.