Gene Expression Data
Assay Details
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Assay
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Reference:
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J:98114
Friedman RA, et al., Eya1 acts upstream of Tbx1, Neurogenin 1, NeuroD and the neurotrophins BDNF and NT-3 during inner ear development. Mech Dev. 2005 May;122(5):625-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5441100
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Gene symbol:
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Bdnf
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Gene name:
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brain derived neurotrophic factor
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Probe:
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BDNF probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 10.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Weak |
Regionally restricted |
6B
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Reduced expression in the ventral domains of both anterior and posteror regions of the otocysts compared to wild type embryos.
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Specimen
6C:
embryonic day 10.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6D:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: inner ear |
Weak |
Regionally restricted |
6D
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Reduced expression in the ventral domains of both anterior and posteror regions of the otocysts compared to wild type embryos.
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Specimen
6E:
embryonic day 12.5
(more )
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6F:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: vestibular component epithelium |
Weak |
Regionally restricted |
6F
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Reduced expression in developing vestibular sensory epithelia (crista ampullaris) compared to wild type mice.
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Specimen
6G:
embryonic day 12.5
(more )
Specimen
6G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6H:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: vestibular component epithelium |
Weak |
Regionally restricted |
6H
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Reduced expression in developing vestibular sensory epithelia (crista ampullaris) compared to wild type mice.
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Specimen
6I:
embryonic day 12.5
(more )
Specimen
6I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6J:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: vestibular component epithelium |
Weak |
Regionally restricted |
6J
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Reduced expression in developing vestibular sensory epithelia (crista ampullaris) compared to wild type mice.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inner ear epithelium |
Weak |
Regionally restricted |
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Authors report reduced expression in both vestibular and cochlear epithelium.
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Analysis Tools