Antibody:	anti Pax2 Z-RX2 (71-6000)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS12: optic sulcus	Present		2A	Expressed in the prospective optic stalk.
TS12: future midbrain	Present		2A
TS12: midbrain-hindbrain junction	Present	Regionally restricted	2A	Expressed in the neural ectoderm of the head fold in the prospective midbrain-hindbrain region.
TS12: future prosencephalon	Present	Regionally restricted	2A	Expressed in the neural plate of the prospective forebrain near the optic placode.

Structure	Level	Pattern	Image	Note
TS12: midbrain-hindbrain junction	Strong		2B	Expressed in the junction between the mes- and metencephalon.

Structure	Level	Pattern	Image	Note
TS12: optic sulcus	Present		2C	Expressed in the prospective optic stalk.
TS12: future midbrain	Present		2C
TS12: future hindbrain	Present	Regionally restricted	2C	Expressed in the neural ectoderm in the prospective hindbrain.

Structure	Level	Pattern	Image	Note
TS13: midbrain-hindbrain junction	Present	Regionally restricted	2D , 2E	Expression extends along the border between the midbrain and hindbrain in a small band of cells of the neuroepithelium.
TS13: optic pit	Present	Regionally restricted	2D , 2E	Expressed in the neural ectoderm of the optic placode. Expression is highest in the ventral half of the optic cup.
TS13: optic eminence	Present	Regionally restricted	2E , 2D	Expressed in the neural ectoderm of the prospective optic stalk that extends from the diencephalon.
TS13: otic placode	Present		2D	Expressed in the auditory vesicle.

Structure	Level	Pattern	Image	Note
TS14: otic pit	Present	Regionally restricted	5A	Expressed in the medial half of the auditory vesicle in the ventral neural ectoderm, adjacent to the neural tube.

Structure	Level	Pattern	Image	Note
TS14: pronephros	Present		5C