Probe:	M2-3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1B	Expression was detected in a broad domain along the neural tube.
TS13: hindgut diverticulum	Present		1B
TS13: lateral plate mesoderm	Present		1B
TS13: somite	Present	Regionally restricted	1B	Expression was detected in the ventral somites.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1E	Expression was detected in a ventral domain.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	1H	Expression was detected along the neural tube.
TS15: 1st branchial arch mandibular component	Strong		1H

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		1K
TS17: sclerotome	Present		1K
TS17: central nervous system	Present	Regionally restricted	1K	Expression flanks the floor plate where Shh is expressed. Expression is strongest at the posterior end.
TS17: limb	Present	Regionally restricted	1K	Expression was detected in the posterior half of each limb bud.
TS17: forebrain	Present	Regionally restricted	1K	Expression was confined predominantly to cells flanking the ventral midline.
TS17: midbrain	Present	Regionally restricted	1K	Expression was confined predominantly to cells flanking the ventral midline.
TS17: diencephalon	Present	Regionally restricted	1K	A dorsal stripe of expression was observed.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	2B	Expression was detected in the posterior half of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	2E	Expression was detected in the posterior half of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Absent		2H	Expression faded from the most posterior region.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 2 mesenchyme	Present	Regionally restricted	3B	Expression was detected in condensing mesenchyme.
TS20: forelimb digit 3 mesenchyme	Present	Regionally restricted	3B	Expression was detected in condensing mesenchyme.
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	3B	Expression was detected in condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit pre-cartilage condensation	Weak		3F

Structure	Level	Pattern	Image	Note
TS21: forelimb digit pre-cartilage condensation	Weak		3J

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit	Present	Regionally restricted	3N	Expression was strongest at the distal tip.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	4B	Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
TS20: hindbrain	Present	Regionally restricted	4B	Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
TS20: midbrain	Present	Regionally restricted	4B	Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
TS20: mandibular process	Present		4B

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4J	Expression was detected in the ventral region but was absent from the midline.
TS19: hindbrain	Present	Regionally restricted	4J	Expression was detected in the ventral region but was absent from the midline.
TS19: midbrain	Present	Regionally restricted	4J	Expression was detected in the ventral region but was absent from the midline.

Structure	Level	Pattern	Image	Note
TS21: gut mesenchyme	Present	Regionally restricted	5B	Expression was detected in the mesoderm in a ring surrounding Shh-expressing cells in the endoderm.

Structure	Level	Pattern	Image	Note
TS20: vibrissa mesenchyme	Present	Regionally restricted	5E	Expression was detected in the mesoderm in a ring around the central ectodermal cells expressing Shh.