Probe:	Brx1b cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present	Regionally restricted	2A	Expression was detected in the lateral mesoderm from the head region.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2B	Expression was detected in the ventral diencephalon and the zone limitans intrathalamica.
TS17: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	2B	Expression was detected in the mandibular and maxillary epithelium.
TS17: 1st branchial arch maxillary component ectoderm	Present	Regionally restricted	2B	Expression was detected in the mandibular and maxillary epithelium.
TS17: trunk mesenchyme	Present	Regionally restricted	2B	Expression was detected in lateral plate mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	2C	Expression was detected in the zona limitans intrathalamica and the mammillary area.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	2D	Expression was detected in the zona limitans intrathalamica and the mammillary area.
TS20: remnant of Rathke's pouch	Present		2D

Structure	Level	Pattern	Image	Note
TS18: diencephalon lateral wall ventricular layer	Present	Regionally restricted	3E	Expression was detected in the lateral (pial) side of the ventricular zone of the zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS18: diencephalon lateral wall ventricular layer	Present	Regionally restricted	3H , 3I	Expression was detected in the lateral side of the ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall ventricular layer	Present	Regionally restricted	3L , 3K	Expression was detected in the lateral side of the ventricular zone.
TS19: diencephalon lateral wall mantle layer	Present		3K , 3L

Structure	Level	Pattern	Image	Note
TS18: diencephalon lateral wall ventricular layer	Present		3P , 3Q	Expression overlapped with Nkx2-2.

Structure	Level	Pattern	Image	Note
TS18: diencephalon	Present	Regionally restricted	4A	Expression was detected in the zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS18: diencephalon	Present	Regionally restricted	4C , 4E	Expression was detected in the zona limitans intrathalamica. There was overlap with Dlx1 expression.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4F	Expression was detected in the zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4J , 4H	Expression was detected in the zona limitans intrathalamica. There was overlap of expression with Arx.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	5A/B	Expression was detected in the zona limitans intrathalamica as it extends toward the lateral surface of the diencephalic wall. Expression was in a narrow path situated on the dorsal side of a broad path containing the spindle-shaped cells (Arx-positive).

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Regionally restricted	6A	Expression was detected in the early embryonic ventral lateral geniculate nucleus. Expression was conserved primarily along the anterioposterior axis.
TS22: diencephalon	Present	Regionally restricted	6A	Expression was detected in the zona limitans intrathalamica near the lateral surface of the diencephalic wall.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Regionally restricted	6E	Expression was detected in the early embryonic ventral lateral geniculate nucleus. Expression was conserved primarily along the anterioposterior axis.
TS22: diencephalon	Present	Regionally restricted	6E	Expression was detected in the zona limitans intrathalamica near the lateral surface of the diencephalic wall.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Regionally restricted	6I'/I" , 6Q , 6U , 6I	Expression was detected in the early embryonic ventral lateral geniculate nucleus. Expression was conserved primarily along the anterioposterior axis.
TS22: diencephalon	Present	Regionally restricted	6I'/I" , 6U , 6I , 6Q	Expression was detected in the zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Regionally restricted	6U , 6T , 6M	Expression was detected in the early embryonic ventral lateral geniculate nucleus. Expression was conserved primarily along the anterioposterior axis.
TS22: diencephalon	Present	Regionally restricted	6M , 6U , 6T	Expression was detected in the zona limitans intrathalamica near the lateral surface of the diencephalic wall.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Present	Regionally restricted	7E/E'	Sections were taken at different coronal levels of embryonic ventral lateral geniculate nucleus. Expression was seen at levels 2 (this section) and 3, but not 1. Expression was seen in the external medullary lamina at levels 2 and 3.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Present	Regionally restricted	7G/G'	Sections were taken at different coronal levels of embryonic ventral lateral geniculate nucleus. Expression was seen at levels 2 and 3 (this section), but not 1. Expression was seen in the external medullary lamina at levels 2 and 3.