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Gene Expression Data
Assay Details
Assay
Reference: J:123586 Fitzgerald DP, et al., Neogenin is expressed on neurogenic and gliogenic progenitors in the embryonic and adult central nervous system. Gene Expr Patterns. 2007 Aug;7(7):784-92
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5440298
Gene symbol: Pcna
Gene name: proliferating cell nuclear antigen
Antibody: Anti-PCNA
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1B/C: embryonic day 14.5 (more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: hippocampus Present Regionally restricted 1B , 1C Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
TS22: telencephalon septum Present Regionally restricted 1B , 1C Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
Specimen 1H/I: embryonic day 14.5 (more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: olfactory cortex ventricular layer Present Regionally restricted 1H , 1I Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
TS22: telencephalon septum Present Regionally restricted 1H , 1I Expression was detected in nuclei of S-phase dividing cells at the basal surface of the ventricular zone.
Specimen 2B: embryonic day 12.5 (more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 2B Expression was detected in dividing cells.
Specimen 2D: embryonic day 14.5 (more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present Regionally restricted 2D Expression was detected in dividing cells in the dorsal midbrain ventricular zone.
Specimen 2I: embryonic day 14.5 (more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: spinal cord ventricular layer Present Regionally restricted 2I Expression was detected in dividing cells.
Specimen 3B/C: embryonic day 17.5 (more )
Note: Double labeled: red - Pcna; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS25: cerebral cortex subventricular zone Present Regionally restricted 3C , 3B Expression was detected in proliferative zones.
TS25: corpus striatum Present Regionally restricted 3C , 3B Expression was detected in proliferative zones.
TS25: hippocampus Present Regionally restricted 3C , 3B Expression was detected in proliferative zones.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory