Antibody:	anti-LIMK1
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS20: maxillary process mesenchyme	Present	Regionally restricted	1A	Expression is in the mesenchyme surrounding the maxillary nerve.

Structure	Level	Pattern	Image	Note
TS21: upper jaw mesenchyme	Present	Regionally restricted	1B	Expression is in the mesenchyme surrounding the maxillary nerve.

Structure	Level	Pattern	Image	Note
TS24: upper jaw mesenchyme	Present	Regionally restricted	1C	Expression is in the mesenchyme surrounding the maxillary nerve.

Structure	Level	Pattern	Image	Note
TS20: nasal cavity epithelium	Present		1D , 1D'
TS20: nose	Present	Regionally restricted	1D' , 1D	Expression is adjacent to the maturing neuroepithelium.

Structure	Level	Pattern	Image	Note
TS21: nasal cavity epithelium	Present		1E , 1E'
TS21: nose	Present	Regionally restricted	1E' , 1E	Expression is adjacent to the maturing neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: nasal cavity epithelium	Present		1F , 1F inset , 1F'
TS24: nose	Present	Regionally restricted	1F' , 1F , 1F inset	Expression is adjacent to the maturing neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Present		1G' , 1G
TS20: gut mesenchyme	Absent		1G' , 1G

Structure	Cell Type	Level	Pattern	Image	Note
TS20: Leydig cell	Leydig cell	Strong		1H' , 1H	Expression is in Leydig cells, absent in other interstitial cells.
TS20: primary sex cord		Weak	Uniform	1H' , 1H

Structure	Level	Pattern	Image	Note
TS21: epidermis	Present	Punctate	2A' , 2A

Structure	Level	Pattern	Image	Note
TS24: epidermis	Strong		2B' , 2B

Structure	Level	Pattern	Image	Note
TS26: epidermis	Strong	Regionally restricted	2C' , 2C	Expression is in the superficial epidermis.
TS26: epidermis basal layer	Absent		2C' , 2C

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region	Present		3A
TS20: forelimb digit	Absent		3A

Structure	Level	Pattern	Image	Note
TS21: hindlimb mesenchyme	Weak		3B , 3B'	Expression is along the dorsal and ventral axis.
TS21: hindlimb musculature	Present		3B' , 3B
TS21: hindlimb interdigital region	Strong	Regionally restricted	3B , 3B'	Expression is distal.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region	Strong		3C , 3C'

Structure	Level	Pattern	Image	Note
TS24: hand epidermis	Weak		3D , 3D'
TS24: forelimb interdigital region	Absent		3D , 3D'

Structure	Level	Pattern	Image	Note
TS20: lens vesicle epithelium	Strong		4A	Absent in lens fibers.
TS20: cornea epithelium	Present		4A
TS20: retina	Present	Regionally restricted	4A	Expression is in the cytoplasm of a subset of cells in the dorsal retina and at lower levels in the ventral retina.

Structure	Level	Pattern	Image	Note
TS20: cornea epithelium	Strong	Regionally restricted	4B , 4B'	In the corneal limbal crypts and the corneal epithelium, expression was detected in an evenly distributed pattern along the whole apical-basal axis.
TS20: epithelium	Weak	Punctate	4B' , 4B	Extra-ocular epithelium.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4C	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4D	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4E	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4F	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: retina	Present		4G	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: retina	Present		4H	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4I	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS21: retina	Absent

Structure	Level	Pattern	Image	Note
TS24: retina	Absent

Structure	Level	Pattern	Image	Note
TS17: heart	Present	Regionally restricted	5A' , 5A	Absent in epicardial cells, present in myocardium.
TS17: cardiac muscle tissue	Present		5A , 5A'

Structure	Level	Pattern	Image	Note
TS20: heart	Present		5B , 5B'	Expression is in myocardium and epicardial cells.
TS20: cardiac muscle tissue	Present		5B , 5B'

Structure	Level	Pattern	Image	Note
TS21: heart	Present		5C' , 5C	Expression is in myocardium and epicardial cells.
TS21: cardiac muscle tissue	Present		5C , 5C'

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Strong		5D' , 5D
TS24: heart	Weak	Regionally restricted	5D' , 5D	Expression is in epicardial cells.

Structure	Level	Pattern	Image	Note
TS17: lung epithelium	Absent		6A' , 6A

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Strong		6B' , 6B
TS20: pleural component	Strong		6B' , 6B

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Strong	Non-Uniform	6C , 6C'	Strong in some parts, lower in others.
TS21: pleural component	Strong		6C , 6C'

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Strong	Non-Uniform	6D' , 6D	Strong in some parts, lower in others.
TS24: pleura	Absent		6D' , 6D

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		6E

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present		6F

Structure	Level	Pattern	Image	Note
TS20: intraembryonic coelom	Strong	Regionally restricted	6G	Expression is in the diaphragm.

Structure	Level	Pattern	Image	Note
TS21: diaphragm	Strong		6H
TS21: lung	Strong		6H

Structure	Level	Pattern	Image	Note
TS24: diaphragm	Weak		6I

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Strong		7A , 7A'
TS17: genitourinary system	Strong	Regionally restricted	7A , 7A'	Expression is in the mesenchyme surrounding the Wolffian duct.

Structure	Level	Pattern	Image	Note
TS17: genitourinary system	Present		7B , 7B'

Structure	Level	Pattern	Image	Note
TS21: early nephron	Present		7C' , 7C
TS21: mesenchyme of metanephros	Present		7C' , 7C

Structure	Level	Pattern	Image	Note
TS24: mesenchyme of metanephros	Present	Regionally restricted	7D , 7D'	Expression is in the mesenchyme surrounding the ureteric buds and in the stroma.
TS24: early nephron	Present		7D' , 7D

Structure	Level	Pattern	Image	Note
TS20: pleural component	Strong		8A
TS20: lung epithelium	Present		8A

Structure	Level	Pattern	Image	Note
TS22: pleural component	Strong		8C
TS22: lung epithelium	Present		8C

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present		8E
TS20: heart	Present	Regionally restricted	8E	Expression is in epicardial cells.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present		8G
TS22: heart	Present	Regionally restricted	8G	Expression is in epicardial cells.

Structure	Level	Pattern	Image	Note
TS20: intraembryonic coelom	Present	Regionally restricted	8I	Expression is in the diaphragm.
TS20: peritoneal component	Present		8I

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Present		8K
TS22: peritoneal component	Present		8K

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present		9A	Expression is down-regulated from the cap mesenchyme surrounding the ureteric bud tips.
TS22: early nephron	Present		9A
TS22: collecting duct	Absent		9A

Structure	Level	Pattern	Image	Note
TS26: mesenchyme of metanephros	Present		9C' , 9C
TS26: nephron	Present		9C' , 9C
TS26: cap mesenchyme	Absent		9C' , 9C

Structure	Level	Pattern	Image	Note
TS26: mesenchyme of metanephros	Present		9D , 9D'
TS26: parietal epithelium of mature renal corpuscle	Weak		9D , 9D'
TS26: visceral epithelium of mature renal corpuscle	Weak		9D , 9D'
TS26: cap mesenchyme	Absent		9D' , 9D

Structure	Level	Pattern	Image	Note
TS26: comma-shaped body	Present		9E , 9E'
TS26: nephron	Present		9E , 9E'
TS26: parietal epithelium of mature renal corpuscle	Weak		9E , 9E'
TS26: visceral epithelium of mature renal corpuscle	Weak		9E , 9E'

Structure	Level	Pattern	Image	Note
TS26: comma-shaped body	Present		9F , 9F'