Gene Expression Data
Assay Details
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Assay
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Reference:
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J:66531
Kraus P, et al., Some distal limb structures develop in mice lacking Sonic hedgehog signaling. Mech Dev. 2001 Jan;100(1):45-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5439625
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Gene symbol:
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Hoxd13
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Gene name:
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homeobox D13
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Probe:
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Hoxd-13 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
(close  )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown:
embryonic day 10.5
(more  )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
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Expression was detected posteriorly in the handplate.
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Specimen
5A:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Weak |
Regionally restricted |
5A
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Weak expression was detected in the posterior hindlimb.
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Specimen
5B:
embryonic day 11.5
(more )
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
5B
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The expression shifts from posterior to anterior.
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Specimen
5C:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D:
embryonic day 12.5
(more )
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
5D
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The expression shifts from posterior to anterior.
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Specimen
5E:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
5E
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Expression was detected in the distalmost part of the hindlimb.
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Specimen
5F:
embryonic day 12.5
(more )
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
5F
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The expression shifts from posterior to anterior.
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Analysis Tools