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Gene Expression Data
Assay Details
Assay
Reference: J:66531 Kraus P, et al., Some distal limb structures develop in mice lacking Sonic hedgehog signaling. Mech Dev. 2001 Jan;100(1):45-58
Assay type: RNA in situ
MGI Accession ID: MGI:5439619
Gene symbol: Ptch1
Gene name: patched 1
Probe: Ptc1 probe5
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen not shown: embryonic day 10.75; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS18: limb Absent
Specimen not shown: embryonic day 11.75; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS19: limb Absent
Specimen 4I: embryonic day 12.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS20: forelimb pre-cartilage condensation Present Regionally restricted 4I Expression was detected in two domains in the forelimb condensing cartilage.
TS20: forelimb Present Regionally restricted 4I An additional expression domain was present more distally in mutant limbs.
Specimen 4J: embryonic day 12.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS20: hindlimb pre-cartilage condensation Present Regionally restricted 4J Expression was detected in two domains in the hindlimb condensing cartilage.
TS20: hindlimb Present Regionally restricted 4J An additional expression domain was present more distally in mutant limbs.
Specimen 4K: embryonic day 13.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS21: forelimb Weak 4K
Specimen 4L: embryonic day 13.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS21: hindlimb Weak 4L
Specimen 4M: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: radius-ulna pre-cartilage condensation Present 4M Expression was detected in the cartilage condensations of the forming zeugopod.
TS20: hand pre-cartilage condensation Present Regionally restricted 4M Expression was detected in the cartilage condensations of the forming metacarpals and slightly weaker domains in the phalange region.
Specimen 4N: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindlimb pre-cartilage condensation Present Regionally restricted 4N Expression was detected in the cartilage condensations of the forming zeugopod.
TS20: foot pre-cartilage condensation Present Regionally restricted 4N Expression was detected in the cartilage condensations of the forming metatarsals and slightly weaker domains in the phalange region.
Specimen 4O: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: forelimb digit pre-cartilage condensation Present 4O
Specimen 4P: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hindlimb digit pre-cartilage condensation Present 4P
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory