Probe:	Notch cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	3A	Highest expression is found in the posterior mesoderm adjacent to the primitive streak.
TS10: primitive streak	Present		3A

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3B	Expression is detected only in the posterior 2/3 of the axial mesoderm. No expression was seen in midline tissues anterior to the node.
TS11: node	Absent		3B

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3C	There is a sharp anterior boundary of expression. Expression is strongest adjacent to the anterior limit of expression.
TS11: embryo ectoderm	Absent		3C

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3D	Expression is just anterior to the node, in two bands on either side of the midline, at right angles to the anterior-posterior axis.
TS11: embryo ectoderm	Absent		3D

Structure	Level	Pattern	Image	Note
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	3E	Expression is present in a somite-sized domain just posterior to the most recently formed somite.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Scattered
TS12: heart	Present	Regionally restricted		Expression is present in the endothelial cells.
TS12: dorsal aorta	Present

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present			Expression is present in the lateral portions of the neural plate.
TS12: future hindbrain	Present	Regionally restricted		Expression is present in the lateral portions of the neural plate at the level of the anterior hindbrain.

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	4A	Expression is present in a somite-sized domain.
TS13: trunk somite	Present	Regionally restricted	4A	Expression is present medially in some anterior somites.
TS13: embryo ectoderm	Present	Regionally restricted	4A	Expression is present in the trigeminal placodes.
TS13: otic placode	Present		4A

Structure	Level	Pattern	Image	Note
TS14: trunk unsegmented mesenchyme	Present	Widespread		In contrast to what was seen earlier, strong expression is seen in most of the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail	Strong		4B
TS15: future spinal cord	Present	Regionally restricted	4B	Expression is present in the dorsalmost spinal cord.
TS15: future hindbrain lateral wall	Present	Regionally restricted	4B	Expression is present cells adjacent to the roof plate.
TS15: embryo ectoderm	Present	Regionally restricted	4B , 4C	Expression is present in the trigeminal, geniculate, petrosum, and nodose placodes.
TS15: otocyst	Present		4C , 4B

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Non-Uniform		Expression is present in the medial or medial and posterior portions of somites, depending on axial level.
TS15: olfactory placode	Present
TS15: dorsal root ganglion	Present			Expression is present in cells immediately lateral to the spinal cord, presumably the neural crest precursors of the dorsal root ganglia.
TS15: arterial system	Present	Regionally restricted		Expression is present in the intersegmental arteries.
TS15: mesonephros	Present			Expression is present in the mesonephric kidney.