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Gene Expression Data
Assay Details
Assay
Reference: J:180644 Wolf XA, et al., Identification of a conserved cis-acting region driving expression of mouse Eomesodermin to the primitive streak, node, and definitive endoderm. Gene Expr Patterns. 2012 Jan;12(1-2):85-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5439009
Gene symbol: Eomes
Gene name: eomesodermin
Antibody: anti-Tbr2 (AB2283)
Detection system: Secondary antibody coupled to Cy3
Assay notes: Confocal section.
Results
Specimen 1A: embryonic day 6.0 (more )
Note: Pre-streak stage.
Structure Level Pattern Image Note
TS8: extraembryonic ectoderm Present 1A
TS8: extraembryonic visceral endoderm Present 1A
Specimen 1B: embryonic day 6.0 (more )
Note: Pre-streak stage. Double labeled: red - Eomes; white - Cdh1 (assay MGI:5439486).
Structure Level Pattern Image Note
TS8: extraembryonic ectoderm Present 1B
TS8: extraembryonic visceral endoderm Present 1B
Specimen 1C: embryonic day 6.0 (more )
Note: Pre-streak stage. Double labeled: red - Eomes; white - Cdh1 (assay MGI:5439486).
Structure Level Pattern Image Note
TS8: extraembryonic ectoderm Present 1C
TS8: extraembryonic visceral endoderm Present 1C
Specimen 1D: embryonic day 6.0 (more )
Note: Pre-streak stage. Double labeled: red - Eomes; white - Cdh1 (assay MGI:5439486).
Structure Level Pattern Image Note
TS8: extraembryonic ectoderm Present 1D
TS8: extraembryonic visceral endoderm Present 1D
Specimen 1E: embryonic day 6.25 (more )
Note: Early streak stage.
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 1E
TS9: extraembryonic visceral endoderm Present 1E
TS9: embryo ectoderm Present Regionally restricted 1E Expression is in the posterior epiblast at the site of streak invagination.
Specimen 1F: embryonic day 6.25 (more )
Note: Early streak stage. Double labeled: red - Eomes; white - Cdh1 (assay MGI:5439486).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 1F
TS9: extraembryonic visceral endoderm Present 1F
TS9: embryo ectoderm Present Regionally restricted 1F Expression is in the posterior epiblast at the site of streak invagination and is absent from a region anterior to the streak.
Specimen 1G: embryonic day 6.25 (more )
Note: Early streak stage.
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 1G
TS9: extraembryonic visceral endoderm Present 1G
TS9: embryo ectoderm Present Regionally restricted 1G Expression is in the posterior epiblast at the site of streak invagination.
Specimen 1H: embryonic day 6.25 (more )
Note: Early streak stage.
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 1H
TS9: extraembryonic visceral endoderm Present 1H
TS9: embryo ectoderm Present Regionally restricted 1H Expression is in the posterior epiblast at the site of streak invagination and is absent from a region anterior to the streak.
Specimen 1I: embryonic day 7.0 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1I
TS10: embryo mesoderm Present 1I
TS10: extraembryonic ectoderm Present 1I
TS10: primitive streak Present Regionally restricted 1I Expression is in the anterior streak.
TS10: anterior visceral endoderm Present 1I
TS10: extraembryonic component Present Regionally restricted 1I Expression is in the chorion.
Specimen 1J: embryonic day 7.0 (more )
Note: Late streak stage. Double labeled: red - Eomes; white - Cdh1 (assay MGI:5439486).
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1J
TS10: embryo mesoderm Present 1J
TS10: extraembryonic ectoderm Present 1J
TS10: primitive streak Present Regionally restricted 1J Expression is in the anterior streak.
TS10: anterior visceral endoderm Present 1J
TS10: extraembryonic component Present Regionally restricted 1J Expression is in the chorion.
Specimen 1K: embryonic day 7.0 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1K
TS10: embryo mesoderm Present 1K
TS10: extraembryonic ectoderm Present 1K
TS10: primitive streak Present Regionally restricted 1K Expression is in the anterior streak.
TS10: anterior visceral endoderm Present 1K
TS10: extraembryonic component Present Regionally restricted 1K Expression is in the chorion.
Specimen 1L: embryonic day 7.0 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1L
TS10: embryo mesoderm Present 1L
TS10: extraembryonic ectoderm Present 1L
TS10: primitive streak Present Regionally restricted 1L Expression is in the anterior streak.
TS10: anterior visceral endoderm Present 1L
TS10: extraembryonic component Present Regionally restricted 1L Expression is in the chorion.
Specimen 1M: embryonic day 7.0 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1M
TS10: embryo mesoderm Present 1M
TS10: extraembryonic ectoderm Present 1M
TS10: primitive streak Present Regionally restricted 1M Expression is in the anterior streak.
TS10: anterior visceral endoderm Present 1M
TS10: extraembryonic component Present Regionally restricted 1M Expression is in the chorion.
Specimen 1N: embryonic day 7.0 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1N
TS10: embryo mesoderm Present 1N
TS10: extraembryonic ectoderm Present 1N
TS10: primitive streak Present Regionally restricted 1N Expression is in the anterior streak.
TS10: anterior visceral endoderm Present 1N
TS10: extraembryonic component Present Regionally restricted 1N Expression is in the chorion.
Specimen 1O: embryonic day 7.0 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1O
TS10: embryo mesoderm Present 1O
TS10: extraembryonic ectoderm Present 1O
TS10: primitive streak Present Regionally restricted 1O Expression is in the anterior streak.
TS10: anterior visceral endoderm Present 1O
TS10: extraembryonic component Present Regionally restricted 1O Expression is in the chorion.
Specimen 1Q: embryonic day 7.0 (more )
Note: Late streak stage. Double labeled: red - Eomes; green - Foxa2 (assay MGI:5439484).
Structure Level Pattern Image Note
TS10: definitive endoderm Present 1Q
TS10: embryo mesoderm Present 1Q
TS10: extraembryonic ectoderm Present 1Q
TS10: primitive streak Present Regionally restricted 1Q
TS10: anterior visceral endoderm Present 1Q
TS10: node Present Regionally restricted 1Q Expression is at the node region at the distal tip of the embryo.
TS10: extraembryonic component Present Regionally restricted 1Q Expression is in the chorion.
Specimen 1R: embryonic day 8.0 (more )
Note: Early headfold stage.
Structure Level Pattern Image Note
TS12: node Present 1R
Specimen 1S: embryonic day 8.0 (more )
Note: Early headfold stage.
Structure Level Pattern Image Note
TS12: node Present 1S
Specimen 1T: embryonic day 8.0 (more )
Note: Early headfold stage.
Structure Level Pattern Image Note
TS12: node Present 1T
TS12: embryo Weak Regionally restricted 1T Expression is in the distal tip mesoderm.
TS12: embryo endoderm Present Regionally restricted 1T Expression is in definitive endoderm.
TS12: extraembryonic ectoderm Strong 1T
Specimen 1U: embryonic day 8.0 (more )
Note: Early headfold stage. Double labeled: red - Eomes; green - T (assay MGI:5439472).
Structure Level Pattern Image Note
TS12: node Present 1U
TS12: embryo Weak Regionally restricted 1U Expression is in the distal tip mesoderm.
TS12: embryo endoderm Present Regionally restricted 1U Expression is in definitive endoderm.
TS12: extraembryonic ectoderm Strong 1U
Specimen 1V: embryonic day 8.0 (more )
Note: Early headfold stage. Double labeled: red - Eomes; green - Cdh1 (assay MGI:5439486).
Structure Level Pattern Image Note
TS12: node Present 1V
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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