Gene Expression Data
Assay Details
Assay
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Reference:
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J:115671
Tanaka TS, et al., Esg1, expressed exclusively in preimplantation embryos, germline, and embryonic stem cells, is a putative RNA-binding protein with broad RNA targets. Dev Growth Differ. 2006 Aug;48(6):381-90
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5438240
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Gene symbol:
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Dppa5a
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Gene name:
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developmental pluripotency associated 5A
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Antibody:
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anti-ESG1
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
2B left:
postnatal
(more )
Specimen
2B left:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Dent's Fixative
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Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
|
2B left
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Expression is present in the cytoplasm.
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Specimen
2B right:
postnatal adult
(more )
Note: The tissue was embedded in polyester wax.
Specimen
2B right:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Paraffin
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Note:
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The tissue was embedded in polyester wax.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: primary oocyte |
Present |
Single cells |
2B right
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Expression is present in the germinal vesicle-stage oocytes.
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Specimen
2C MII + Sperm:
embryonic day 0.5
(more )
Note: This embryo was in the process of being fertilized.
Specimen
2C MII + Sperm:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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Note:
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This embryo was in the process of being fertilized.
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Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
|
2C MII + Sperm
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Expression is present in the cytoplasm, although the signal is relatively reduced in the area surrounding the metaphase II chromosomes.
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Specimen
2C Ana:
embryonic day 0.5
(more )
Note: This embryo is in second anaphase.
Specimen
2C Ana:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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Note:
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This embryo is in second anaphase.
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Specimen
2C Telo:
embryonic day 0.5
(more )
Note: This embryo is in second telophase.
Specimen
2C Telo:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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Note:
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This embryo is in second telophase.
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Specimen
2D Un:
postnatal
(more )
Specimen
2D Un:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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Structure |
Level |
Pattern |
Image |
Note |
TS28: secondary oocyte |
Present |
Spotted |
2D Un
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Expression is present in the cytoplasm, although the signal is relatively reduced in the area surrounding the metaphase II chromosomes.
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Specimen
2D 1:
embryonic day 0.5
(more )
Specimen
2D 1:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Dent's Fixative
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Specimen
2D 2:
embryonic day 1.0
(more )
Specimen
2D 2:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Dent's Fixative
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Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
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2D 2
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There is comparable staining in all blastomeres.
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Specimen
2D 4:
embryonic day 2.0
(more )
Specimen
2D 4:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Dent's Fixative
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Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Present |
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2D 4
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There is comparable staining in all blastomeres.
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Specimen
2D Mo:
embryonic day 3.0
(more )
Specimen
2D Mo:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Dent's Fixative
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Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Non-Uniform |
2D Mo
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Staining is weaker in the outer cell layer.
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Specimen
2D Bl:
embryonic day 3.5
(more )
Specimen
2D Bl:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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