Gene Expression Data
Assay Details
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Assay
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Reference:
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J:98391
Taketo T, et al., Expression of SRY proteins in both normal and sex-reversed XY fetal mouse gonads. Dev Dyn. 2005 Jun;233(2):612-22
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5438005
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Gene symbol:
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Amh
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Gene name:
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anti-Mullerian hormone
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Antibody:
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anti-MIS
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
4a/b:
embryonic day 11.8
(more  )
Note: 22 tail somites. The authors identified this specimen as XY. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
Specimen
4a/b:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.8
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Sex:
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Male
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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22 tail somites. The authors identified this specimen as XY. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Present |
Regionally restricted |
4b
,
4a
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Expression is predominantly in the central region near the mesonephros. The staining is cytoplasmic.
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Specimen
4d/e/f:
embryonic day 12.3
(more )
Note: 25 tail somites. The authors identified this specimen as XY. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
Specimen
4d/e/f:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.3
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Sex:
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Male
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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25 tail somites. The authors identified this specimen as XY. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: Sertoli cell |
Sertoli cell |
Strong |
Non-Uniform |
4d
,
4e
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Expression is seen in the cytoplasm of Sertoli cells within the testis cords near the medial/central region.
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| TS20: gonad |
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Present |
Regionally restricted |
4d
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4f
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Expression is seen less frequently seen near the posterior pole.
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Specimen
7c/d:
embryonic day 12.4
(more )
Note: 25 tail somites. The authors identified this specimen as XY. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
Specimen
7c/d:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.4
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Sex:
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Male
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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25 tail somites. The authors identified this specimen as XY. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: testis |
Strong |
Widespread |
7d
,
7c
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Expression is present in the cytoplasm of most cells.
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Specimen
7e/f:
embryonic day 13.4; X/ SryPOS-TIR
(more )
Note: This specimen is an ovatestis; testis is present in 60% of the gonad. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
Specimen
7e/f:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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embryonic day 13.4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This specimen is an ovatestis; testis is present in 60% of the gonad. Double labeled: red - Amh; green - Sry (assay MGI:5438002).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gonad |
Present |
Regionally restricted |
7f
,
7e
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Expression is present in clusters in the central region.
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Specimen
7g/h:
embryonic day 14.4; X/ SryPOS-TIR
(more )
Specimen
7g/h:
(close )
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Genetic Background:
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C57BL/6-Chr YTIR
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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embryonic day 14.4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: gonad |
Present |
Regionally restricted |
7g
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Expression is present in clusters in the central region.
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| TS22: primary sex cord |
Strong |
Non-Uniform |
7h
,
7g
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Expression is intense in the well-organized testis cords, less intense in the partially organized testis cords.
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Analysis Tools