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Gene Expression Data
Assay Details
Assay
Reference: J:98391 Taketo T, et al., Expression of SRY proteins in both normal and sex-reversed XY fetal mouse gonads. Dev Dyn. 2005 Jun;233(2):612-22
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5437996
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Antibody: anti-SRY (Mab #15)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen Not shown: embryonic day 10.4 (more )
Note: 10 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS17: gonad primordium Absent Expression was absent in 3 of 3 gonads examined.
Specimen 2a: embryonic day 10.6 (more )
Note: 11 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS17: gonad primordium Absent 2a Expression was lacking in 2 of 5 gonads examined. (In another 2 there was faint staining that was not convincingly higher than the background staining.)
Specimen 2b/3a: embryonic day 10.6 (more )
Note: 11 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS17: gonad primordium Weak Scattered 2b , 3a Cells with faint staining are scattered over both poles in 1 of 5 gonads examined. It is diffuse and only partially concentrated in the nuclei. It is seen over the nuclei of the cells surrounding some, but not all, germ cells.
TS17: mesonephros Absent 2b
Specimen 2c: embryonic day 10.8 (more )
Note: 12 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS18: gonad primordium Weak Widespread 2c Expressing cells are scattered over the genital ridge.
Specimen 2d: embryonic day 11.3 (more )
Note: 14 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Strong Widespread 2d Stained cells occupy the entire genital ridge.
TS19: mesonephros Absent 2d
Specimen 2e: embryonic day 11.5 (more )
Note: 17 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Strong Widespread 2e Stained cells occupy the entire genital ridge.
TS19: mesonephros Absent 2e
Specimen 2f: embryonic day 11.8 (more )
Note: 23 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Strong Widespread 2f Stained cells occupy the entire genital ridge.
TS19: mesonephros Absent 2f
Specimen 2g: embryonic day 12.3 (more )
Note: 25 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS20: testis Present Scattered 2g In this gonad expressing cells are present at the anterior pole (pictured), as well as the posterior pole. This was the only gonad at this stage observed to have this staining pattern.
Specimen 2h: embryonic day 12.3 (more )
Note: 25 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS20: testis Present Scattered 2h Expressing cells are limited to the posterior pole in 3 of 5 gonads examined at this stage.
Specimen 2i: embryonic day 12.3 (more )
Note: 25 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS20: primary sex cord Present Scattered 2i
Specimen 2j: embryonic day 11.6 (more )
Note: 21 tail somites. The authors identified this specimen as XX.
Structure Level Pattern Image Note
TS19: gonad Absent 2j
Specimen 2k: embryonic day 11.5 (more )
Note: 17 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Present 2k Expression is limited to the genital ridge (seen at higher magnification in the inset).
TS19: dorsal aorta Absent 2k
TS19: future spinal cord Absent 2k
TS19: heart ventricle Absent 2k
TS19: liver Absent 2k
TS19: somite Absent 2k
TS19: tongue Absent 2k
Specimen 2l: embryonic day 11.6 (more )
Note: 19 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Strong 2l
Specimen 3b: embryonic day 11.3 (more )
Note: 14 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 3b Expressing cells cluster in the genital ridge; there is no expression in the surface epithelium.
TS19: mesonephros Absent 3b
TS19: primordial germ cell of gonad Absent 3b
Specimen 3c: embryonic day 11.8 (more )
Note: 23 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 3c Most somatic cells are expressing, whereas the surface epithelium and tunica albuginia do not stain.
TS19: mesonephros Absent 3c
TS19: primordial germ cell of gonad Absent 3c
Specimen 3d: embryonic day 11.8 (more )
Note: 23 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 3d Expressing cells cluster in the genital ridge where germ cells are scarce.
Specimen 3e: embryonic day 12.3 (more )
Note: 25 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS20: primary sex cord Weak Single cells 3e The intense staining in the interstitium is non-specific.
Specimen 6a: embryonic day 11.3 (more )
Note: 12 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Weak Scattered 6a A small number of expressing cells are scattered over the genital ridge.
Specimen 6b: embryonic day 11.4 (more )
Note: 14 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Present Widespread 6b Stained cells occupy the entire genital ridge.
TS19: mesonephros Absent 6b
Specimen 6c: embryonic day 11.8 (more )
Note: 18 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS19: gonad Present Widespread 6c Stained cells occupy the entire genital ridge.
TS19: mesonephros Absent 6c
Specimen 6d: embryonic day 12.3 (more )
Note: 24 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS20: testis Weak Widespread 6d Weakly stained cells occupy the entire genital ridge.
Specimen 6e: embryonic day 12.4 (more )
Note: 25 tail somites. The authors identified this specimen as XY.
Structure Level Pattern Image Note
TS20: primary sex cord Trace Regionally restricted 6e Expression is present at the posterior pole.
Specimen 6f: embryonic day 11.4; X/SryPOS-TIR (more )
Note: 14 tail somites.
Structure Level Pattern Image Note
TS19: gonad Absent 6f
Specimen 6g: embryonic day 11.4; X/SryPOS-TIR (more )
Note: 15 tail somites. This specimen is undifferentiated; no testis cords are seen.
Structure Level Pattern Image Note
TS19: gonad Present Non-Uniform 6g Staining is delayed in these mutants relative to wild type. Expressing cells are scattered over the genital ridge but are more abundant at both poles.
Specimen 6h: embryonic day 11.5; X/SryPOS-TIR (more )
Note: 17 tail somites. This specimen is undifferentiated; no testis cords are seen.
Structure Level Pattern Image Note
TS19: gonad Strong Widespread 6h Stained cells occupy the entire genital ridge.
Specimen 6i: embryonic day 11.8; X/SryPOS-TIR (more )
Note: 25 tail somites. This specimen is undifferentiated; no testis cords are seen.
Structure Level Pattern Image Note
TS19: gonad Strong Widespread 6i Stained cells occupy the entire genital ridge.
Specimen 6j: embryonic day 12.3; X/SryPOS-TIR (more )
Note: 27 tail somites. This specimen is undifferentiated; no testis cords are seen.
Structure Level Pattern Image Note
TS20: gonad Strong Widespread 6j Stained cells occupy the entire genital ridge.
Specimen 6k: embryonic day 12.4; X/SryPOS-TIR (more )
Note: 28 tail somites. This specimen is undifferentiated; no testis cords are seen.
Structure Level Pattern Image Note
TS20: gonad Present Widespread 6k Stained cells occupy the entire genital ridge. Staining was present in 7 of 7 gonads examined at this stage.
Specimen 6l: embryonic day 12.4; X/SryPOS-TIR (more )
Note: 28 tail somites. This specimen is an ovatestis; testis cords are seen in the central 20% of the gonad.
Structure Level Pattern Image Note
TS20: gonad Present Widespread 6l Stained cells occupy the entire genital ridge. Staining was present in 7 of 7 gonads examined at this stage.
Specimen Not shown: embryonic day 12.4; X/SryPOS-TIR (more )
Note: 30 tail somites.
Structure Level Pattern Image Note
TS20: ovary Absent At this stage, there was no expression in the 4 specimens with ovaries.
TS20: gonad Absent At this stage, there was no expression in the 6 specimens with ovotestes.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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