Bmp4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:39658 Buscher D, et al., Evidence for genetic control of Sonic hedgehog by Gli3 in mouse limb development. Mech Dev. 1997 Mar;62(2):175-82
Assay type:	RNA in situ
MGI Accession ID:	MGI:5437947
Gene symbol:	Bmp4
Gene name:	bone morphogenetic protein 4

Probe:	Bmp4 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

5C left ©

Specimen C left: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		5C left
TS17: limb	Present	Regionally restricted	5C left	Expressed in posterior, anterior and apical ectodermal ridge regions.

5C middle ©

Specimen C middle: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present		5C middle
TS19: limb	Present	Regionally restricted	5C middle	Expressed in posterior, anterior and apical ectodermal ridge regions.

5C right ©

Specimen C right: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	5C right	Restricted to areas of condensation and an anterior region that possibly participates in the development of the humerus.
TS20: limb pre-cartilage condensation	Present		5C right

5D left ©

Specimen D left: embryonic day 10.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		5D left	Expression is the same as in the wild-type control.
TS17: limb	Present	Regionally restricted	5D left	Expression is the same as in the wild-type control. Expressed in posterior, anterior and apical ectodermal ridge regions.

5D middle ©

Specimen D middle: embryonic day 11.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present		5D middle	Expression is the same as in the wild-type control.
TS19: limb	Present	Regionally restricted	5D middle	Expression is the same as in the wild-type control. Expressed in posterior, anterior and apical ectodermal ridge regions.

5D right ©

Specimen D right: embryonic day 12.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS20: limb pre-cartilage condensation	Present		5D right
TS20: limb	Present	Regionally restricted	5D right	Expressed in the areas of condensation, an anterior region that possibly participates in the development of the humerus, and an additional most anterior domain.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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